Abstract
Long-term exposure to fluoxetine produces a desensitization of hypothalamic postsynaptic 5-hydroxytryptamine (5-HT)1Areceptors, indicated by a substantial inhibition of the 5-HT1A receptor-mediated stimulation of oxytocin and adrenocorticotropic hormone (ACTH) secretion. The present study investigated the time course and mechanism of this desensitization after discontinuation of fluoxetine administration. Male rats were injected with saline or fluoxetine (10 mg/kg/day, i.p.) for 14 days and were challenged with a 5-HT1A agonist, [8-hydroxy-2-(dipropylamino)tetralin (8-OH-DPAT) 50 μg/kg, s.c.] 2, 4, 7, 14, 28, or 60 days post-treatment. In control animals, 8-OH-DPAT significantly increased (approximately 15-fold) plasma levels of oxytocin and ACTH. At 2 days post-treatment, oxytocin and ACTH responses to 8-OH-DPAT were reduced by 74% and 68%, respectively. During further withdrawal from fluoxetine, there was a gradual increase in the oxytocin response toward control levels. However, even 60 days after discontinuation of fluoxetine, the oxytocin response was still significantly reduced by 26% compared with controls. In contrast, the suppressed ACTH response to 8-OH-DPAT (a less-sensitive indicator of desensitization) gradually returned to control levels by day 14 of withdrawal from fluoxetine. Interestingly, the sustained reductions in the hormone responses occurred in the absence of reductions in Gz or Gi protein levels in the hypothalamus. Furthermore, this desensitization was sustained in the absence of detectable levels of fluoxetine and norfluoxetine in plasma and brain tissue. These findings suggest that the sustained desensitization of hypothalamic 5-HT1Areceptor systems, observed during fluoxetine withdrawal, may be due to altered interactions among the protein components of the 5-HT1A receptor system, rather than their absolute levels.
Footnotes
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Send reprint requests to: Louis D. Van de Kar, Department of Pharmacology, Stritch School of Medicine, Loyola University of Chicago, 2160 S. First Ave., Maywood IL 60153.E-mail:lvandek{at}luc.edu
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↵1 This study was supported in part by U.S. Public Health Service Grant NS34153 (to L.D.V.dK.). A portion of this study was presented as a poster at the Society for Neuroscience meeting (Soc Neurosci Abstr 23, 385.5, 1997).
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↵2 Current address: Biological Psychiatry and Research Service, Hines Veterans Administration Medical Center, Hines, IL 60141.
- Abbreviations:
- 5-HT
- 5-hydroxytryptamine (serotonin)
- 8-OH-DPAT
- 8-hydroxy-2-(dipropylamino)tetralin
- ACTH
- adrenocorticotropic hormone
- ANOVA
- analysis of variance
- CRH
- corticotrophin-releasing hormone
- HPLC
- high-pressure liquid chromatography
- IOD
- integrated optical density
- SSRI
- selective serotonin reuptake inhibitor
- Received May 1, 1998.
- Accepted September 1, 1998.
- The American Society for Pharmacology and Experimental Therapeutics
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