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Research ArticleArticle

Cellular Mechanisms of Renal Adaptation of Sodium Dependent Sulfate Cotransport to Altered Dietary Sulfate in Rats

Kazuko Sagawa, Debra C. DuBois, Richard R. Almon, Heini Murer and Marilyn E. Morris
Journal of Pharmacology and Experimental Therapeutics December 1998, 287 (3) 1056-1062;
Kazuko Sagawa
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Debra C. DuBois
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Richard R. Almon
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Heini Murer
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Marilyn E. Morris
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Abstract

The renal transport and fractional reabsorption of inorganic sulfate is altered under conditions of sulfate deficiency or excess. The objective of this study was to examine the cellular mechanisms of adaptation of renal sodium/sulfate cotransport after varying dietary intakes of a sulfur containing amino acid, methionine. Female Lewis rats were divided into four groups and fed diets containing various concentrations of methionine (0, 0.3, 0.82 and 2.46%) for 8 days. Urinary excretion rates and renal clearance of sulfate were significantly decreased in the animals fed a 0% methionine diet or a 0.3% methionine diet, and significantly increased in the animals fed a 2.46% methionine diet when evaluated on days 4 and 7. Serum sulfate concentrations were unchanged by diet treatment in all animals. The fractional reabsorption of sulfate was significantly increased in the animals fed the 0% methionine diet and the 0.3% methionine diets, and decreased in the animals fed the 2.46% methionine diet. Increased mRNA and protein levels for the sodium/sulfate transporter (NaSi-1) were found in the kidney cortex following treatment with the 0 and 0.3% methionine diet groups. Sulfate homeostasis by renal reabsorption is maintained by an up-regulation of steady state levels of NaSi-1 mRNA and protein when the diet is low in methionine.

Footnotes

  • Send reprint requests to: Dr. Marilyn E. Morris, 527 Hochstetter Hall, Department of Pharmaceutics, State University of New York at Buffalo, Amherst, NY 14260.

  • ↵1 This work was supported by NSF Grant IBN 9629470, a grant from the Western New York Kidney Foundation/Upstate NY Transplant Services, a multidisciplinary grant from the Research Foundation of State University of New York and a grant from the Kapoor Foundation of State University of New York at Buffalo (M.E.M.). Additional support was provided by National Institutes of Health Grant AG 10629 (R.R.A.) and Swiss National Science Foundation (H.M.).

  • Abbreviations:
    BBM
    brush border membrane
    BLM
    basolateral membrane
    GFR
    glomerular filtration rate
    mRNA
    messenger RNA
    RT-PCR
    reverse transcriptase-polymerase chain reaction
    ELISA
    enzyme-linked immunosorbent assay
    DPH
    1,6-diphenyl-1,3,5-hexatriene
    NaSi-1
    sodium/sulfate cotransport
    SSC
    standard saline citrate
    PBS
    phosphate-buffered saline
    • Received April 8, 1998.
    • Accepted July 5, 1998.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics
Vol. 287, Issue 3
1 Dec 1998
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Research ArticleArticle

Cellular Mechanisms of Renal Adaptation of Sodium Dependent Sulfate Cotransport to Altered Dietary Sulfate in Rats

Kazuko Sagawa, Debra C. DuBois, Richard R. Almon, Heini Murer and Marilyn E. Morris
Journal of Pharmacology and Experimental Therapeutics December 1, 1998, 287 (3) 1056-1062;

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Research ArticleArticle

Cellular Mechanisms of Renal Adaptation of Sodium Dependent Sulfate Cotransport to Altered Dietary Sulfate in Rats

Kazuko Sagawa, Debra C. DuBois, Richard R. Almon, Heini Murer and Marilyn E. Morris
Journal of Pharmacology and Experimental Therapeutics December 1, 1998, 287 (3) 1056-1062;
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