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Abstract

Constitutive and inducible nitric oxide synthases in human megakaryoblastic cells.

R Lelchuk, M W Radomski, J F Martin and S Moncada
Journal of Pharmacology and Experimental Therapeutics September 1992, 262 (3) 1220-1224;
R Lelchuk
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M W Radomski
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J F Martin
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S Moncada
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Abstract

Human megakaryoblastic cells (Meg-01) were found to possess constitutive and express inducible nitric oxide (NO) synthase activities. The constitutive NO synthase was Ca+(+)- and NADPH-dependent, as is the NO synthase found previously in human platelets. Stimulation of Meg-01 cells by the cytokines interleukin-1 beta (0.15-32.5 ng/ml) and tumor necrosis factor-alpha (0.15-10 ng/ml) resulted in expression of the inducible, Ca+(+)-independent, NO synthase. This activity was increased by the addition of NADPH, tetrahydrobiopterin and sepiapterin as cofactors. Induction of this enzyme was accompanied by a decrease in the constitutive NO synthase activity, a phenomenon which was prevented or reversed by dexamethasone (1 microM). Thus, human early differentiated megakaryocytic cells can synthesize NO from L-arginine by both the constitutive and the inducible NO synthases. These findings indicate that these enzymes may play an important biological role in megakaryocyte and platelet functions.

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Journal of Pharmacology and Experimental Therapeutics
Vol. 262, Issue 3
1 Sep 1992
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Abstract

Constitutive and inducible nitric oxide synthases in human megakaryoblastic cells.

R Lelchuk, M W Radomski, J F Martin and S Moncada
Journal of Pharmacology and Experimental Therapeutics September 1, 1992, 262 (3) 1220-1224;

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Abstract

Constitutive and inducible nitric oxide synthases in human megakaryoblastic cells.

R Lelchuk, M W Radomski, J F Martin and S Moncada
Journal of Pharmacology and Experimental Therapeutics September 1, 1992, 262 (3) 1220-1224;
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