Abstract
A study has been made of the secretory response of the isolated, Lockeperfused adrenal gland of the dog to angiotensin and bradykinin, using an automated trihydroxyindole fluorometric method for the estimation of epinephrine and norepinephrine. Both angiotensin and bradykinin were found to be extremely potent liberators of adrenal catecholamines. The response to the peptides was dose-dependent and rapid in onset. Both angiotensin and bradykinin caused a statistically significant increase in the proportions of epinephrine in the augmented catecholamine output. Perfusion of the gland with a calciumfree Locke's solution resulted in a reduced secretory response to angiotensin. During perfusion with calcium-free Locke's solution there was a concomitant decrease in both the spontaneous output and in the proportion of epinephrine in the spontaneous output.
Footnotes
- Received May 31, 1966.
- Accepted November 11, 1966.
- © 1967 by The Williams & Wilkins Company
JPET articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|