Abstract
The effect of cardiac glycosides on the Ca++ uptake of cardiac sarcoplasmic reticulum fragments was investigated employing the Millipore filtration method. It was found that both ouabain and strophanthin inhibited the Ca++ uptake of isolated cardiac sarcoplasmic reticulum fragments, and that the optimal concentration for this inhibitory effect was about 10-6 M. This inhibitory effect of the cardiac glycosides was more marked in the NaCl medium than in the KCl medium. The determination of the time course of Ca++ uptake indicated that the inhibition of Ca++ uptake by the cardiac glycosides was most marked at the initial 0.5- to 1-min period of incubation, and became less as the incubation time was prolonged.
Footnotes
- Accepted January 31, 1966.
- The Williams & Wilkins Comapny
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