Abstract
A gas chromatographic method has been adapted for the analysis of acetylcholine in rat brain. Rat brains were frozen, extracted in acid ethanol, flash evaporated, taken up in water, reacted with borohydride and the resulting ethanol estimated on a gas chromatograph. The compounds considered possible interfering substances in the bioassay do not interfere with the estimation of acetylcholine by the chemical method. Acetylcholine levels measured by the chemical method and by bioassay are similar.
Footnotes
- Accepted June 17, 1965.
- The Williams & Wilkins Comapny
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