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Journal of Pharmacology And Experimental Therapeutics Fast Forward
First published on August 25, 2005; DOI: 10.1124/jpet.105.090167

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Received for publication May 31, 2005.
Revised August 11, 2005.
Accepted for publication August 16, 2005.

EGCG Impairs Chemokine Production in Human Colon Epithelial Cell Lines

Debora Porath 1, Christoph Riegger 1, Juergen Drewe 2, Joseph Schwager 1*

1 DSM Nutritional Products Ltd. 2 University Hospital, Univ. of Basel

* Address correspondence to: E-mail: joseph.schwager{at}dsm.com

Abstract

A major component in green tea, epigallocatechin-3-gallate (EGCG), is reported to interfere with different steps of a number of inflammatory pathways. After oral administration, EGCG is retained in the gastro- intestinal tract, where it is thought to exert preventive functions against inflammatory bowel disease (IBD) and colon cancer. In this study, the human colon adenocarcinoma cell lines HT29 and T84 were used to investigate the effect of EGCG on intestinal inflammation. HT29 and T84 cells were stimulated with tumor necrosis factor-alpha (TNF-{alpha}) to induce the "inflammatory" condition and to trigger the inflammatory cascade in vitro, and treated with EGCG to study its effect on inflammatory processes. The secretion of the chemokines interleukin (IL)-8 and macrophage inflammatory protein (MIP)-3{alpha} and prostaglandin E2 (PGE2) was determined by ELISA. The gene expression level was measured by quantitative real time (RT)-PCR. Treatment of TNF-{alpha}-stimulated HT29 cells with EGCG dose-dependently inhibited the synthesis of IL-8, MIP-3{alpha} and PGE2. Treatment with EGCG also inhibited the production of IL-8 and MIP-3{alpha} in TNF-{alpha}-stimulated T84 cells. Gene expression analysis in both HT29 and T84 cells revealed that EGCG down-regulates genes involved in inflammatory pathways. This study shows that EGCG acts broadly on the production of chemokines and PGE2 in the chemokine and eicosanoid pathways of gastro-intestinal epithelial cells. Therefore, EGCG might prove useful for the prevention and/or attenuation of colonic disorders.


Key words: EGCG, ELISA, chemokines, epithelial cells, gene expression, intestine


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