JPET Introducing ALZET?ew Model 2006 Pump

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

Journal of Pharmacology And Experimental Therapeutics Fast Forward
First published on February 1, 2005; DOI: 10.1124/jpet.104.081315

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jpet.104.081315v1
jpet.104.081315v2
313/3/1272    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oz, M.
Right arrow Articles by Zhang, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oz, M.
Right arrow Articles by Zhang, L.


Received for publication November 23, 2004.
Revised January 27, 2005.
Accepted for publication January 31, 2005.

ADDITIVE EFFECTS OF ENDOGENOUS CANNABINOID ANANDAMIDE AND ETHANOL ON {alpha}7-NICOTINIC ACETYLCHOLINE RECEPTOR-MEDIATED RESPONSES IN XENOPUS OOCYTES

Murat Oz 1*, Shelley Jackson 1, Amina Woods 1, Marisela Morales 1, Li Zhang 2

1 NIH/NIDA/IRP, Cellular Neurobiology Branch 2 NIH/NIAAA, Lab. Mol. Cellular Neurobiology

* Address correspondence to: E-mail: moz{at}intra.nida.nih.gov

Abstract

The interaction between the effects of the endogenous cannabinoid receptor agonist anandamide and ethanol on the function of homomeric {alpha}7-nicotinic acetylcholine (nACh) receptors expressed in Xenopus oocytes were investigated using the two-electrode voltage-clamp technique. Anandamide and ethanol reversibly inhibited currents evoked with ACh (100 ìM) in a concentration-dependent manner. Coapplication of anandamide and ethanol caused a significantly greater inhibition of {alpha}7- nACh receptor function than anandamide or ethanol alone. The IC50 value of 238 ± 34 nM for anandamide inhibition decreased significantly to 104 ± 23 nM in the presence of 30 mM ethanol. The inhibition of {alpha}7-mediated currents by coapplication of anandamide and ethanol was not altered by phenylmethylsulfonyl fluoride, an inhibitor of anandamide hydrolyzing enzyme, or AM404, an anandamide-transport inhibitor. Analysis of oocytes by matrix-assisted laser desorption/ionization technique indicated that ethanol treatment did not alter the lipid profile of oocytes and there is negligible, if any, anandamide present in these cells. Results of studies with chimeric {alpha}7-nACh-5-HT3 receptors comprised of the amino-terminal domain of the {alpha}7-nACh receptor and the transmembrane and carboxyl-terminal domains of 5-HT3 receptors suggest that while ethanol inhibition of the {alpha}7-nAChR is likely to involve the N-terminal region of the receptor, the site of action for anandamide is located in the transmembrane and carboxyl-terminal domains of the receptors. These data indicate that endocannabinoids and ethanol potentiate each other's inhibitory effects on {alpha}7-nACh receptor function through distinct regions of the receptor.


Key words: anandamide, drug abuse, endocannabinoid, ethanol, ligand-gated receptor, nicotinic receptor


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
U. Baranowska, M. Gothert, R. Rudz, and B. Malinowska
Methanandamide Allosterically Inhibits in Vivo the Function of Peripheral Nicotinic Acetylcholine Receptors Containing the {alpha}7-Subunit
J. Pharmacol. Exp. Ther., September 1, 2008; 326(3): 912 - 919.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
C. E. Spivak, C. R. Lupica, and M. Oz
The Endocannabinoid Anandamide Inhibits the Function of {alpha}4beta2 Nicotinic Acetylcholine Receptors
Mol. Pharmacol., October 1, 2007; 72(4): 1024 - 1032.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
N. Hejazi, C. Zhou, M. Oz, H. Sun, J. H. Ye, and L. Zhang
{Delta}9-Tetrahydrocannabinol and Endogenous Cannabinoid Anandamide Directly Potentiate the Function of Glycine Receptors
Mol. Pharmacol., March 1, 2006; 69(3): 991 - 997.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2005 by the American Society for Pharmacology and Experimental Therapeutics.