JPET Medipak's UVLI- Bags blind and protect

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

Journal of Pharmacology And Experimental Therapeutics Fast Forward
First published on May 17, 2004; DOI: 10.1124/jpet.104.069153

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jpet.104.069153v1
311/1/164    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adamson, C. R
Right arrow Articles by Morkin, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adamson, C. R
Right arrow Articles by Morkin, E.


Received for publication March 30, 2004.
Revised May 17, 2004.
Accepted for publication May 17, 2004.

Regulation of Gene Expression in Cardiomyocytes by Thyroid Hormone and Thyroid Hormone Analogs, 3,5-Diiodothyropropionic Acid and CGS 23425

Cynthia R Adamson 1*, Niranjan Maitra 1, Joseph John Bahl 1, Kevin Greer 1, Scott Klewar 1, James Hoying 1, Eugene Morkin 1

1 University of Arizona

* Address correspondence to: E-mail: cadamson{at}u.arizona.edu

Abstract

The heart is an important target of thyroid hormone actions. Only a limited number of cardiac target genes have been identified and little is known about their regulation by T3 and thyroid hormone analogs. We used an oligonucleotide microarray to identify novel cardiac genes regulated by T3 and two thyroid hormone analogs, 3,5-diidodothyropropionic acid (DITPA) and CGS 23425. DITPA binds with lower affinity than T3 to thyroid hormone receptor {alpha}1 and {beta}1 isoforms whereas CGS 23425 binds selectively to {beta}1. Fluorescent-labeled cDNA was prepared from cultured heart cells maintained in medium stripped of thyroid hormone ('hypothyroid' control) or treated with T3, DITPA and CGS 23425 at concentrations 5X their respective Kd values for 48 h. The arrays were scanned and analyzed using an ANOVA program. Sixty-four genes were identified that were >1.5 times up or down regulated by one of the treatments with P<0.05. The genes regulated by T3 and DITPA were nearly identical. Thirteen genes were differentially regulated by CGS 23425. Genes encoding contractile proteins, Ca++-ATPase of sarcoplasmic reticulum and several proteins of mitochondrial oxidative phosphorylation were up regulated by T3 and DITPA but not by CGS 23425. These results indicate that the some, but not all of the actions of thyroid hormone analogs can be explained by differences in gene activation.


Key words: CGS 23425, Thyroid hormone, Thyroid hormone analogs, diiodothyropropionic acid, gene expression, microarray


This article has been cited by other articles:


Home page
 EndocrinologyHome page
S. A. Mousa, L. O'Connor, F. B. Davis, and P. J. Davis
Proangiogenesis Action of the Thyroid Hormone Analog 3,5-Diiodothyropropionic Acid (DITPA) Is Initiated at the Cell Surface and Is Integrin Mediated
Endocrinology, April 1, 2006; 147(4): 1602 - 1607.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2004 by the American Society for Pharmacology and Experimental Therapeutics.