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Journal of Pharmacology And Experimental Therapeutics Fast Forward
First published on May 18, 2004; DOI: 10.1124/jpet.104.067066

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Received for publication February 18, 2004.
Revised April 23, 2004.
Accepted for publication May 18, 2004.

Expression and induction of cytochrome P450s in rat white adipose tissue

Kouichi YOSHINARI 1, Takeshi SATO 1, Nao OKINO 1, Junko SUGATANI 1, Masao Miwa 1*

1 University of Shizuoka

* Address correspondence to: E-mail: miwa{at}u-shizuoka-ken.ac.jp

Abstract

Lipophilic environmental pollutants are often stored in adipose tissues after exposure. These compounds have been well studied in terms of their cell toxicity in organs such as liver and kidney, and their xenoestrogenic action on reproductive tissues as endocrine disruptors. However, the effects of these chemicals on the depot, adipose tissue, have not been studied, although adipose tissue is an important endocrine tissue secreting obesity/diabetes-related hormones and cytokines. In this study, we identified the expression of cytochrome P450s in rat white adipose tissues and investigated the effects of typical lipophilic cytochrome P450 inducers, namely phenobarbital, dexamethasone and {beta}-naphthoflavone. The results showed that {beta}-naphthoflavone was a strong CYP1A inducer in adipose tissue as well as in liver. It increased CYP1A1 mRNA, protein and its related activity, ethoxyresorufin O-deethylase. Phenobarbital and dexamethasone also induced both the mRNA and protein of CYP2Bs and CYP3As, respectively, in adipose tissue, although significant inter-individual differences were observed. Furthermore, we demonstrated that 48 hr fasting was as effective in adipose tissue as in the liver in the induction of CYP2E1 mRNA and protein. These results suggest that the mechanisms by which cytochrome P450 genes are regulated in the liver are also functional in rat adipose tissues. This has raised the possibility that lipophilic environmental contaminants accumulated in adipose tissue may dysregulate thegene expression profile.


Key words: Aryl hydrocarbon receptor, adipose tissue, cytochrome P450, environmental pollutant, fasting, rat


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