A Trimeric Anti-HER2/neu ScFv and Tumor Necrosis Factor- Fusion Protein Induces HER2/neu Signaling and Facilitates Repair of Injured Epithelia

Data Supplement

Files in this Data Supplement:

• Data Supplement - Sup. 1. Construction of anti-human HER2/neu ScFv (C6MH3-B1)- TNF-á and anti-dansyl ScFv-TNF-á. Sup. 2. Anti-HER2/neu ScFv-TNF-á and anti-HER2/neu ScFv-TNF-á(S147Y) demonstrate anti-apoptotic activity in the presence of Actinomycin D. Sup. 3. Anti-HER2/neu ScFv-TNF-á (A) and anti-HER2/neu ScFv-TNF-á(S147Y) (B) induce tyrosine phosphorylation of HER2/neu. Sup. 4. Activation of p44/42 MAPK (ERK1+2) or Akt induced by anti-HER2/neu ScFv-TNF-á was inhibited by U0126 or LY294002.