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Journal of Pharmacology And Experimental Therapeutics, Vol. 92, Issue 4, 425-431, 1948
Copyright © 1948 by American Society for Pharmacology and Experimental Therapeutics

AN IMPROVED COLORIMETRIC METHOD FOR THE ESTIMATION OF HISTAMINE

SANFORD M. ROSENTHAL 1 and HERBERT TABOR 1

1 Division of Physiology, National Institute of Health, Bethesda, Md.

A colorimetric method for the detection of histamine has been developed. It has been adapted for the determination of free and conjugated histamine in animal tissues and body fluids.

Preliminary results upon various tissues, and with histaminase, indicate a satisfactory degree of specificity.

The sensitivity of the method is approximately 0.5 microgram.

Submitted on November 28, 1947




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Copyright © 1948 by the American Society for Pharmacology and Experimental Therapeutics.