A DIURETIC FACTOR PRESENT IN NORMAL DOG, HUMAN, AND DIALYZED HUMAN URINE

¹ From the Department of Physiology and Pharmacology, The Bowman Gray School of Medicine of Wake Forest College, Winston-Salem, N. C.

1. It has been demonstrated that when normal human and dog urine and dialyzed human urine are infused intravenously into normal unanesthetized dogs, in amounts equivalent to 0.5-1.0% of the body weight, there results a marked increase in the rate of urine flow over the pre-injection rate. The volume of urine recovered in excess of that infused plus that which would have been excreted without diuresis represents a reduction in total body water of the animal equivalent to approximately 1.0-1.5% of the body weight.

2. The infusion of an identical volume of control solution, which had the same sodium chloride and urea concentration as the corresponding urine, resulted in an increased rate of urine flow which was considerably less than that resulting from the urine infusion. The volume of urine recovered in excess of that which would have been excreted without infusion was equal to the volume infused. The infusion of the control solution did not decrease the total body water of the animal.

3. The infusion of a volume of distilled water equal to the volume of infused dialyzed human urine resulted in a slight increase in the rate of urine flow in one experiment. In five experiments the rate of urine flow was unaffected. The total body water of the animal was increased by the infusion of this volume of distilled water.

4. It is concluded that normal human and dog urine contain a non-dialyzable diuretic substance.