THE SPECIFICITY OF SEX HORMONES ON THE TISSUE ALDEHYDE SHIFT IN THE RAT KIDNEY AND OF FUCHSIN SULFUROUS ACID REAGENT ON ALDEHYDES

¹ Research Laboratories of McKesson & Robbins, Inc., Bridgeport, Connecticut

1. Various influences were tested for their possible effect on the tissue aldehyde content of the rat kidney intercortico-medullary zone, administration of androgenic substances, of antidiuretic and diuretic compounds, of certain electrolytes and stimulation of metabolism. Of these, only androgens effect a deposition of tissue aldehydes in the aldehyde depleted ICM zone.

2. By utilizing the procedure of successive alkalinization and acidification, or acidification alone, proof has been offered that the FSA stainable substances in tissues are true tissue aldehydes and not ketones.

3. The work of a group of investigators using phenylhydrazine to demonstrate ketosteroids in tissues may be reinterpreted in the light of the present findings.