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Journal of Pharmacology And Experimental Therapeutics, Vol. 87, Issue 2, 159-168, 1946
Copyright © 1946 by American Society for Pharmacology and Experimental Therapeutics

AN EVALUATION OF THE RHODAMINE B METHOD FOR THE DETERMINATION OF ANTIMONY

ALFRED GELLHORN 1, M. E. KRAHL 1, and JOHN W. FERTIG 1

1 From the Department of Pharmacology, College of Physicians and Surgeons, Columbia University, and the Department of Biostatistics, School of Public Health of the Faculty of Medicine, Columbia University

A technique for the quantitative determination of small quantities of antimony with rhodamine B has been described in detail. The precision of the method has been evaluated by making repeated determinations of antimony in the range from 2.0 to 15.0 micrograms and these results have been analyzed statistically. It has been found that within the above range the standard deviation is 0.4 micrograms of antimony. Thus, the maximum error of the technique (2.5 S.D. units) is ± 1.0 micrograms of antimony.

The recovery of four organic antimonials from liver has been compared with their recovery from aqueous solutions and it has been found that the presence of tissue does not interfere with the determinations.

The disadvantages and advantages of the Rhodamine B method for antimony determination have been discussed.

Submitted on April 5, 1946






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Copyright © 1946 by the American Society for Pharmacology and Experimental Therapeutics.