THE REDUCTION OF NEOPRONTOSIL BY TISSUES IN VITRO

¹ From The Department of Physiology and Pharmacology, Duke University School of Medicine, Durham, North Carolina

1. A suspension of rat liver incubated semi-anaerobically with neoprontosil reduced the dye. Kidney under the same conditions causes comparatively little reduction and brain and muscle none at all.

2. The addition of cystine, thioglycollic acid or ethyl alcohol increases the reduction rate of liver but has little effect when added to kidney or brain. Glucose, lactic acid, amino acids, amines, succinic acid, choline, aldehydes and glutathione have no effect on the rate of reduction by liver.

3. Cysteine and thioglycollic acid alone or in the presence of copper or iron salts reduce neoprontosil only very slowly.

4. Boiling or the addition of KCN prevents the reduction by the liver alone and with the substrates. Washed liver reduces very slowly and the addition of cysteine and thioglycollic acid increases the reduction rate.

5. Changing the pH from 6.7 to 7.8 has little effect on the reduction rates with the exception of alcohol which reduces more rapidly at the higher pH.

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