JPET

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by KRAUSE, R. F.
Right arrow Articles by RILEY, R. F.
Right arrow Search for Related Content
PubMed
Right arrow Articles by KRAUSE, R. F.
Right arrow Articles by RILEY, R. F.
Journal of Pharmacology And Experimental Therapeutics, Vol. 71, Issue 3, 287-292, 1941
Copyright © 1941 by American Society for Pharmacology and Experimental Therapeutics

THE RELIABILITY OF THE COBALT-ISOPROPYLAMINE COLOR REACTION FOR AMYTAL THE EVALUATION OF CHROMOGENIC SUBSTANCES IN URINE

R. F. KRAUSE 1 and RICHARD F. RILEY 1

1 From the Department of Biochemistry and Pharmacology, School of Medicine and Dentistry, The University of Rochester, Rochester, New York

1. The cobalt-isopropylamine color reaction for the determination of amytal has been studied critically. Optimal amounts of reagents are recommended which reduce the method error to less than 2 per cent for concentrations of amytal of 20-300 mgm. per cent.

2. Dog urine normally contains pigments which are extracted by chloroform and which are included in the total color of the amytal reaction. This factor has been accounted for.

3. Dog urine normally also contains non-barbiturate chromogenic substances extracted by chloroform which produce color in the amytal reaction. An attempt has been made to estimate this factor quantitatively.

4. Dogs fed amytal excrete, in the urine, an increased amount of color producing substances. This increase cannot be taken as proof of amytal excretion.

Submitted on December 3, 1940






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1941 by the American Society for Pharmacology and Experimental Therapeutics.