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Journal of Pharmacology And Experimental Therapeutics, Vol. 71, Issue 3, 246-252, 1941
Copyright © 1941 by American Society for Pharmacology and Experimental Therapeutics

THE ESTIMATION BY A MANOMETRIC METHOD OF THE ACTIVITY OF CHOLINESTERASE IN LYMPH

DALE G. FRIEND 1 and OTTO KRAYER 1

1 From the Department of Pharmacology, Harvard Medical School

1. A manometric method for the determination of the activity of cholinesterase in body fluids and tissue extracts is described. Of the species of animals examined, the activity of cholinesterase is highest in the serum of horse and man, lower in the dog, and lowest in the cat.

2. The cholinesterase activity of thoracic lymph and cervical lymph of dogs, per cubic centimeter, is distinctly less than the cholinesterase activity of serum.

3. Prostigmine methylsulfate given by mouth or by intravenous injection inhibits the activity of the cholinesterase in thoracic and cervical lymph.

Submitted on December 14, 1940






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