QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: jpet.106.104620v1 319/3/1104    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Serna, H. Articles by Vergara, P. Search for Related Content PubMed PubMed Citation Articles by Serna, H. Articles by Vergara, P.

GASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Mast Cell Stabilizer Ketotifen [4-(1-Methyl-4-piperidylidene)-4H-benzo[4,5]cyclohepta[1,2-b]thiophen-10(9H)-one Fumarate] Prevents Mucosal Mast Cell Hyperplasia and Intestinal Dysmotility in Experimental Trichinella spiralis Inflammation in the Rat

Department of Cell Biology, Physiology, and Immunology, Universitat Autònoma de Barcelona, Bellaterra, Spain

Trichinella spiralis infection in rats induces hypermotility and an abnormal response to cholecystokinin (CCK) similar to motor disturbances observed in irritable bowel syndrome. Mast cell hyperplasia is also characteristic of this experimental model. The aim of our study was to correlate mast cell activity with the development of dysmotility and to demonstrate whether the mast cell stabilizer ketotifen [4-(1-methyl-4-piperidylidene)-4H-benzo[4,5]cyclohepta[1,2-b]thiophen-10(9H)-one fumarate] could prevent the development of intestine hypermotility. Sprague-Dawley rats were infected with T. spiralis and, 5 days after infection, treated with the mast-cell stabilizer ketotifen (10 mg/kg/day). Twelve days after infection, intestinal spontaneous motor activity and response to CCK were evaluated by means of strain-gauge transducers. Immunohistochemistry for rat mast cell protease II (RMCPII), cyclooxygenase (COX)-2, and inducible nitric-oxide synthase (iNOS) was performed in intestinal specimens. In addition, RMCPII and myeloperoxidase were determined in serum. Infected control rats showed hypermotility, mast cell hyperplasia, increased RMCPII levels, increased myeloperoxidase, and overexpression of COX-2 and iNOS. In contrast, ketotifen-treated rats showed spontaneous intestinal motility and CCK response similar to the noninfected control rats. Mast cell hyperplasia and RMCPII were reduced in ketotifen-treated rats. Inflammatory parameters were less modified by ketotifen, but those animals that received the longest ketotifen treatment showed a slight amelioration in these parameters. These results indicate that mast cells are implicated in the development of hypermotility. The treatment with ketotifen prevented hypermotility and mast cell hyperplasia and diminished mucosal mast cell activity.

Address correspondence to: Dr. Patri Vergara, Unidad de Fisiologia, Facultad de Veterinaria, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. E-mail: patri.vergara{at}uab.es

This article has been cited by other articles:

				S. Khaldi, G. Gargala, L. Le Goff, S. Parey, A. Francois, J. Fioramonti, J.-J. Ballet, J.-P. Dupont, P. Ducrotte, and L. Favennec Cryptosporidium parvum Isolate-Dependent Postinfectious Jejunal Hypersensitivity and Mast Cell Accumulation in an Immunocompetent Rat Model Infect. Immun., November 1, 2009; 77(11): 5163 - 5169. [Abstract] [Full Text] [PDF]

				E. Li, A. Zhao, T. Shea-Donohue, and S. M. Singer Mast Cell-Mediated Changes in Smooth Muscle Contractility during Mouse Giardiasis Infect. Immun., September 1, 2007; 75(9): 4514 - 4518. [Abstract] [Full Text] [PDF]

				R. Roka, A. Ait-Belgnaoui, C. Salvador-Cartier, R. Garcia-Villar, J. Fioramonti, H. Eutamene, and L. Bueno Dexamethasone prevents visceral hyperalgesia but not colonic permeability increase induced by luminal protease-activated receptor-2 agonist in rats Gut, August 1, 2007; 56(8): 1072 - 1078. [Abstract] [Full Text] [PDF]

				C. B. Collins, J. McGrath, A. W. Baird, and D. P. Campion Effect of Mast Cell Degranulation on Chicken Ileal Ion Transport In Vitro Poult. Sci., May 1, 2007; 86(5): 843 - 849. [Abstract] [Full Text] [PDF]