QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: jpet.106.103358v1 318/1/373    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alonso-Escolano, D. Articles by Radomski, M. W. Search for Related Content PubMed PubMed Citation Articles by Alonso-Escolano, D. Articles by Radomski, M. W.

CELLULAR AND MOLECULAR

Protein Kinase C Mediates Platelet-Induced Breast Cancer Cell Invasion

Department of Integrative Biology and Pharmacology and Institute of Molecular Medicine for the Prevention of Human Diseases, University of Texas, Houston, Texas (D.A.-E., C.M., K.C., A.R., P.J., M.J.S.-M., T.J., P.R., M.W.R.); School of Pharmacy Trinity College Dublin, Ireland (C.M., M.W.R.); and Silesian Center for Heart Diseases, Zabrze, Poland (M.W.R.)

Platelets play an important role in carcinogenesis, but the underlying molecular mechanisms remain poorly understood. To investigate the effects of platelets on in vitro invasion of MCF7 human breast cancer cells, human MCF7 cells were used to study their interactions with platelets using aggregometry and cell invasion chambers. Zymography and quantitative polymerase chain reaction (PCR) were used to study matrix metalloproteinases (MMPs), whereas Western blot was used to study protein kinase C (PKC) in MCF7 cells. We observed that platelets promoted invasion of MCF7 cells (3-fold increase, p < 0.05, n = 3) and that this process correlated with a dramatic increase in MMP-9 (8 fold-increase, p < 0.001, n = 3), which is known to facilitate cancer cell invasion. Because both platelets and MCF7 cells have been shown to release MMP-9, we investigated the cellular source that accounted for this increase. The time course and the use of specific protein synthesis inhibitors demonstrated that most of the increase in MMP-9 levels derived from de novo synthesis of this protease by cancer cells. Furthermore, platelets activated PKC in MCF7 cells after 1 h of incubation (18.45 ± 4.75% increase, p < 0.05, n = 4-7), which, in turn, led to an up-regulation of MMP-9 mRNA (from 60 ± 20 to 1040 ± 100 pg, p < 0.001, n = 3) and protein levels (18-fold increase, p < 0.001, n = 3), with the subsequent cell invasion-promoting effects. PKC plays a crucial role in transducing the invasion-promoting effects of platelets in breast cancer cells, and the specific inhibition of PKC may be a strategy to decrease platelet-mediated cancer cell invasion.

Address correspondence to: Marek W. Radomski, Chair of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, University of Dublin Trinity College, Dublin, Ireland. E-mail: radomskm{at}tcd.ie

This article has been cited by other articles:

				S. K. Park, Y. S. Hwang, K.-K. Park, H.-J. Park, J. Y. Seo, and W.-Y. Chung Kalopanaxsaponin A inhibits PMA-induced invasion by reducing matrix metalloproteinase-9 via PI3K/Akt- and PKC{delta}-mediated signaling in MCF-7 human breast cancer cells Carcinogenesis, July 1, 2009; 30(7): 1225 - 1233. [Abstract] [Full Text] [PDF]

				C.-L. Chen and H.-C. Chen Functional suppression of E-cadherin by protein kinase C{delta} J. Cell Sci., February 15, 2009; 122(4): 513 - 523. [Abstract] [Full Text] [PDF]

				J. Villar, M. I. Arenas, C. M. MacCarthy, M. J. Blanquez, O. M. Tirado, and V. Notario PCPH/ENTPD5 Expression Enhances the Invasiveness of Human Prostate Cancer Cells by a Protein Kinase C{delta} Dependent Mechanism Cancer Res., November 15, 2007; 67(22): 10859 - 10868. [Abstract] [Full Text] [PDF]

				J.-S. Wang, C.-Y. Chang, S.-E. Chow, Y.-W. Chen, and C.-M. Yang Exercise modulates platelet-nasopharyngeal carcinoma cell aggregation and subsequent tissue factor and matrix metalloproteinase activities J Appl Physiol, September 1, 2007; 103(3): 763 - 770. [Abstract] [Full Text] [PDF]