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CARDIOVASCULAR

Clovamide-Type Phenylpropenoic Acid Amides, N-Coumaroyldopamine and N-Caffeoyldopamine, Inhibit Platelet-Leukocyte Interactions via Suppressing P-Selectin Expression

Phytonutrients Laboratory (J.B.P.) and Nutrient Requirements and Functions Laboratory (N.S.), Beltsville Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland

N-Coumaroyldopamine and N-caffeoyldopamine are clovamide-type phenylpropenoic acid amides found in Theobroma cacao. In this article, N-coumaroyldopamine and N-caffeoyldopamine were investigated to determine their effects on P-selectin expression and platelet-leukocyte interactions in vitro and in vivo models. At the concentration of 0.05 µM, they were able to inhibit P-selectin expression on the platelets by 33 (P < 0.011) and 30% (P < 0.012), respectively. The inhibition was partially blocked by ₂-adrenoceptor antagonists, suggesting that ₂ receptors are probably engaged in the inhibition. N-Caffeoyldopamine and N-coumaroyldopamine could also suppress platelet-leukocyte interactions in blood samples by 36 (P < 0.013) and 32% (P < 0.011), respectively, at the same concentration (0.05 µM). In an animal study, mice administrated orally with N-caffeoyldopamine (50 and 100 µg/35 g of body weight) also showed great reduction in the P-selectin expression and platelet-leukocyte interactions by 31 to 45% (P < 0.011) and 34 to 43% (P < 0.014), respectively. These data suggest that the clovamide-type phenylpropenoic acid amides are able to suppress platelet-leukocyte interactions via inhibiting P-selectin expression.

Address correspondence to: Jae B. Park, Phytonutrients Laboratory, BH-NRC, ARS, USDA, Beltsville, MD 20705. E-mail: parkj{at}ba.ars.usda.gov

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