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CARDIOVASCULAR

K_v Channels Contribute to Nitric Oxide- and Atrial Natriuretic Peptide-Induced Relaxation of a Rat Conduit Artery

Department of Chemical Pharmacology (Y.T., K.K.) and Department of Pharmacology (K.T., K.O., K.S.), Toho University School of Pharmaceutical Sciences, Funabashi, Japan; and Department of Anesthesiology, Division of Molecular Medicine, (G.T., M.E., M.S., K.N., E.S., L.T.), Department of Molecular & Medical Pharmacology (L.T.), Department of Physiology (E.S.), Cardiovascular Research Laboratory (E.S., L.T.), and Brain Research Institute (E.S., L.T.), David Geffen School of Medicine at UCLA, Los Angeles, California

The role of K⁺ channels in nitric oxide (NO)-induced vasorelaxation has been largely investigated in resistance vessels where iberiotoxin-sensitive MaxiK channels play a predominant role. However, the nature of the K⁺ channel(s) involved in the relaxation triggered by NO-releasing compounds [nitroglycerin, NTG; NOR 3 [(±)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide]] or atrial natriuretic peptide (ANP) in the conduit vessel aorta has remained elusive. We now demonstrate that, in rat aorta, the relaxation due to these vasorelaxants is not affected by the MaxiK channel blocker iberiotoxin (10^–7–10^–6 M) as was the control vascular bed used (mesenteric artery). The inability of iberiotoxin to prevent NO/ANP-induced aortic relaxations was not due to lower expression of MaxiK in aorta or due to the predominance of iberiotoxin-resistant channels in this conduit vessel. Aortic relaxations were strongly diminished by 4-aminopyridine (4-AP) (5 x 10^–3 M) or by tetraethylammonium (>2 x 10^–3 M) at concentrations known to inhibit voltage-dependent K⁺ (K_v) 2-type channels but not by other K⁺ channel inhibitors, glibenclamide, apamin, charybdotoxin, tertiapin, or E-4031 N-[4-[[1-[2-(6-methyl-2-pyridinyl)ethyl]-4-piperidinyl-]carbonyl]phenyl]methanesulfonamide dihydrochloride). Consistent with a role of K_v2-type channels, K_v currents in A7r5 aortic myocytes were stimulated by NTG and inhibited by 5 x 10^–3 M 4-AP. Furthermore, immunocytochemistry, immunoblot, and real-time polymerase chain reaction analyses confirmed the presence of K_v2.1 channels in aorta. K_v2.1 transcripts were 100-fold more abundant than K_v2.2. Our results support low-affinity 4-AP-sensitive K_v channels, assembled at least partially by K_v2.1 subunit, as downstream effectors of NO/ANP-signaling cascade regulating aortic vasorelaxation and further demonstrate vessel-specific K⁺ channel involvement in NO/ANP-induced relaxation.

Address correspondence to: Dr. Yoshio Tanaka, Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences, 2-2-1 Miyama, Funabashi-City, Chiba 274-8510, Japan. E-mail: yotanaka{at}phar.toho-u.ac.jp