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CELLULAR AND MOLECULAR
2-Adrenergic Receptors Promotes Surface Expression and Functional Activity of 
1D-Adrenergic Receptors
Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia
The 
1D-adrenergic receptor (1D-AR) is a G protein-coupled receptor (GPCR) that is poorly trafficked to the cell surface and largely nonfunctional when heterologously expressed by itself in a variety of cell types. We screened a library of approximately 30 other group I GPCRs in a quantitative luminometer assay for the ability to promote 1D-AR cell surface expression. Strikingly, these screens revealed only two receptors capable of inducing robust increases in the amount of 1D-AR at the cell surface: 1B-AR and 
2-AR. Confocal imaging confirmed that coexpression with 2-AR resulted in translocation of 1D-AR from intracellular sites to the plasma membrane. Additionally, coimmunoprecipitation studies demonstrated that 1D-AR and 2-AR specifically interact to form heterodimers when coexpressed in HEK-293 cells. Ligand binding studies revealed an increase in total 1D-AR binding sites upon coexpression with 2-AR, but no apparent effect on the pharmacological properties of the receptors. In functional studies, coexpression with 2-AR significantly enhanced the coupling of 1D-AR to norepinephrine-stimulated Ca2+ mobilization. Heterodimerization of 2-AR with 1D-AR also conferred the ability of 1D-AR to cointernalize upon 2-AR agonist stimulation, revealing a novel mechanism by which these different adrenergic receptor subtypes may regulate each other's activity. These findings demonstrate that the selective association of 1D-AR with other receptors is crucial for receptor surface expression and function and also shed light on a novel mechanism of cross talk between 1- and 2-ARs that is mediated through heterodimerization and cross-internalization.
Address correspondence to: Randy A. Hall, Department of Pharmacology, Emory University School of Medicine, 5113 Rollins Research Center, 1510 Clifton Rd., Atlanta, GA 30322. E-mail: rhall{at}pharm.emory.edu
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