QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: jpet.104.072546v1 311/3/1241    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Suzuki, Y. Articles by Bani, D. Search for Related Content PubMed PubMed Citation Articles by Suzuki, Y. Articles by Bani, D.

INFLAMMATION AND IMMUNOPHARMACOLOGY

Inhibition of Poly(ADP-Ribose) Polymerase Prevents Allergen-Induced Asthma-Like Reaction in Sensitized Guinea Pigs

Departments of Pediatrics (Y.S.), Neuroscience and Vision (A.C., H.S.), University of Verona, Verona, Italy; Departments of Preclinical and Clinical Pharmacology (E.M., C.M.), Anatomy, Histology, and Forensic Medicine (D.B.), University of Firenze, Firenze, Italy; and Institute of Pharmacology, University of Messina, Messina, Italy (S.C.)

Poly(ADP-ribose) polymerase (PARP) plays an important role in tissue injury in conditions associated with oxidative stress and inflammation. Because asthma is a chronic inflammatory disorder of the airways, we designed the present experimental study to evaluate the effects of PARP inhibition on allergen-induced asthma-like reaction in ovalbumin-sensitized guinea pigs. Cough and dyspnea in response to ovalbumin aerosol were absent in naive guinea pigs, whereas they became severe in the sensitized animals. In the latter ones, ovalbumin aerosol also induced a rapid increase in PARP activity, bronchiolar constriction, pulmonary air space inflation, mast cell degranulation, poly(ADP-ribose) and nitrotyrosine immunostaining, myeloperoxidase activity, and malondialdehyde in lung tissue, as well as a rise in the amounts of nitrites and tumor necrosis factor- in bronchoalveolar lavage fluid. Pretreatment with the PARP inhibitors 3-aminobenzamide (10 mg/kg b.wt.) or 5-aminoisoquinolinone (0.5 mg/kg b.wt.) given i.p. 3 h before ovalbumin challenge significantly reduced the severity of cough and the occurrence of dyspnea and delayed the onset of respiratory abnormalities. Both PARP inhibitors were also able to prevent the above morphological and biochemical changes of lung tissue or bronchoalveolar lavage fluid induced by ovalbumin challenge. Conversely, p-aminobenzoic acid, the inactive analog of 3-aminobenzamide, had no effects.

Address correspondence to: Daniele Bani, Department of Anatomy, Histology, and Forensic Medicine, Section of Histology, University of Florence, Viale G. Pieraccini, 6, I-50139 Florence, Italy. E-mail: daniele.bani{at}unifi.it

This article has been cited by other articles:

				E. Masini, L. Giannini, S. Nistri, L. Cinci, R. Mastroianni, W. Xu, S. A. A. Comhair, D. Li, S. Cuzzocrea, G. M. Matuschak, et al. Ceramide: a Key Signaling Molecule in a Guinea Pig Model of Allergic Asthmatic Response and Airway Inflammation J. Pharmacol. Exp. Ther., February 1, 2008; 324(2): 548 - 557. [Abstract] [Full Text] [PDF]

				A. Aldinucci, G. Gerlini, S. Fossati, G. Cipriani, C. Ballerini, T. Biagioli, N. Pimpinelli, L. Borgognoni, L. Massacesi, F. Moroni, et al. A Key Role for Poly(ADP-Ribose) Polymerase-1 Activity during Human Dendritic Cell Maturation J. Immunol., July 1, 2007; 179(1): 305 - 312. [Abstract] [Full Text] [PDF]

				O. Mustapha, R. Datta, K. Oumouna-Benachour, Y. Suzuki, C. Hans, K. Matthews, K. Fallon, and H. Boulares Poly(ADP-ribose) Polymerase-1 Inhibition Prevents Eosinophil Recruitment by Modulating Th2 Cytokines in a Murine Model of Allergic Airway Inflammation: A Potential Specific Effect on IL-5 J. Immunol., November 1, 2006; 177(9): 6489 - 6496. [Abstract] [Full Text] [PDF]

				D. Bani, L. Giannini, A. Ciampa, E. Masini, Y. Suzuki, M. Menegazzi, S. Nistri, and H. Suzuki Epigallocatechin-3-Gallate Reduces Allergen-Induced Asthma-Like Reaction in Sensitized Guinea Pigs J. Pharmacol. Exp. Ther., June 1, 2006; 317(3): 1002 - 1011. [Abstract] [Full Text] [PDF]