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CHEMOTHERAPY, ANTIBIOTICS, AND GENE THERAPY

Involvement of Estrone-3-Sulfate Transporters in Proliferation of Hormone-Dependent Breast Cancer Cells

Department of Molecular Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science (T.N., M.S., K.T., H.Y., T.M., I.T.), Noda, Japan; Department of Pharmaceutical Biology, Faculty of Pharmaceutical Sciences, Kanazawa University (T.N., A.T.), Kanazawa, Japan; and GenoMembrane, Inc. (H.Y.), Tsurumi, Kanagawa, Japan

Although circulating estrone-3-sulfate is a major precursor of biologically active estrogen, permeation across the plasma membrane is unlikely to occur by diffusion because of the high hydrophilicity of the molecule. The object of this study was to clarify the involvement of specific transporter(s) in the supply of estrone-3-sulfate to human breast cancer-derived T-47D cells, which grow in an estrogen-dependent manner. The proliferation of T-47D cells was increased by the addition of estrone-3-sulfate, or estradiol, to the cultivation medium. The initial uptake rate of estrone-3-sulfate kinetically exhibited a single saturable component, with K_m and V_max values of 7.6 µM and 172 pmol/mg of protein/min, respectively. The replacement of extracellular Na⁺ with Li⁺, K⁺, or N-methylglucamine⁺ had no effect on the uptake of [³H]estrone-3-sulfate. The uptake was strongly inhibited by sulfate conjugates of steroid hormones, but not by estradiol-17-glucuronide. Taurocholate and sulfobromophthalein inhibited the uptake, whereas other tested anionic and cationic compounds did not. The expression of organic anion transporting polypeptides, OATP-D and OATP-E, which are candidate transporters of estrone-3-sulfate, was detected by reverse transcription-polymerase chain reaction analysis, although their actual involvement in the uptake of estrogen remains to be clarified. In conclusion, the uptake of estrone-3-sulfate by T-47D cells was mediated by a carrier-mediated transport mechanism, suggesting that the estrogen precursor is actively imported by estrogen-dependent breast cancer cells.

Address correspondence to: Dr. Ikumi Tamai, Department of Molecular Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamasaki, Noda, Chiba 278-8510, Japan. E-mail: tamai{at}rs.noda.tus.ac.jp