QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: jpet.104.070755v1 311/2/634    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lavoie, J.-C. Articles by Chessex, P. Search for Related Content PubMed PubMed Citation Articles by Lavoie, J.-C. Articles by Chessex, P.

TOXICOLOGY

Interaction between Ascorbate and Light-Exposed Riboflavin Induces Lung Remodeling

Pediatrics Department, Sainte-Justine Hospital, University of Montreal, Montreal, Quebec, Canada (J.C.L., T.R.); and Division of Neonatology, Children's and Women's Health Centre of British Columbia, Vancouver, British Columbia, Canada (P.C.)

Light-exposed parenteral multivitamins induce in lungs peroxide-like oxidant responses as well as the initiation of fibrosis. We hypothesized that peroxides generated in light-exposed total parenteral nutrition (TPN) affect lung remodeling. The objective was to assess the specific roles of peroxides, multivitamin preparation (MVP), and light exposure on lung remodeling during TPN. Three-day-old guinea pigs fitted with an indwelling catheter were assigned to the following intravenous regimens: TPN or MVP ± photoprotection, H₂O₂± glutathione, MVP ± metabisulfite, or ascorbic acid ± riboflavin. Fed animals served as controls. After 4 days, lungs were sampled to determine alveolarization (intercepts), -actin mRNA (protection assay), and apoptosis (terminal deoxynucleotidyl transferase dUTP nick-end labeling). Data were analyzed by analysis of variance. The infusion of light-exposed multivitamins induced a 20% lower (p < 0.01) alveolarization index than fed controls, and 3-fold higher (p < 0.01) apoptotic events. This was prevented by photoprotecting TPN. The effect of multivitamins on the alveolarization index was reproduced (p < 0.05) by infusion of light-exposed riboflavin in the presence of vitamin C. The alveolarization index correlated (r² = 0.35; p < 0.05) with -actin mRNA, suggesting alveolar disruption. Antiperoxides conferred no protection against decreased alveolarization. Lung remodeling induced by exposure of TPN to ambient light is not due to a direct effect of infused peroxides but rather to an interaction between vitamin C and peroxides generated by the exposure of riboflavin to light. It is speculated that this interaction may play a role in the development of chronic lung disease of premature infants who receive TPN and have immature antioxidant defenses.

Address correspondence to: Dr. Jean-Claude Lavoie, Research Centre, Sainte-Justine Hospital, 3175 Côte Ste-Catherine, Montreal, QC, Canada H3T 1C5. E-mail: jclavoie{at}justine.umontreal.ca

This article has been cited by other articles:

				R. Sherlock and P. Chessex Shielding Parenteral Nutrition From Light: Does the Available Evidence Support a Randomized, Controlled Trial? Pediatrics, June 1, 2009; 123(6): 1529 - 1533. [Abstract] [Full Text] [PDF]

				M. Khashu, A. Harrison, V. Lalari, J.-C. Lavoie, and P. Chessex Impact of shielding parenteral nutrition from light on routine monitoring of blood glucose and triglyceride levels in preterm neonates Arch. Dis. Child. Fetal Neonatal Ed., March 1, 2009; 94(2): F111 - F115. [Abstract] [Full Text] [PDF]