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TOXICOLOGY

Roles of Necrosis, Apoptosis, and Mitochondrial Dysfunction in S-(1,2-Dichlorovinyl)-L-cysteine Sulfoxide-Induced Cytotoxicity in Primary Cultures of Human Renal Proximal Tubular Cells

Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan (L.H.L., D.A.P., S.E.H.) and Department of Comparative Biosciences, University of Wisconsin School of Veterinary Medicine, Madison, Wisconsin (R.J.K., A.A.E.)

S-(1,2-Dichlorovinyl)-L-cysteine (DCVC) is the penultimate nephrotoxic metabolite of the environmental contaminant trichloroethylene. Although metabolism of DCVC by the cysteine conjugate -lyase is the most studied bioactivation pathway, DCVC may also be metabolized by the flavin-containing monooxygenase (FMO) to yield DCVC sulfoxide (DCVCS). Renal cellular injury induced by DCVCS was investigated in primary cultures of human proximal tubular (hPT) cells by assessment of time- and concentration-dependent effects on cellular morphology, acute cellular necrosis, apoptosis, mitochondrial function, and cellular glutathione (GSH) status. Confluent hPT cells incubated with as little as 10 µM DCVCS for 24 h exhibited morphological changes, although at least 100 µM DCVCS was required to produce marked changes. Acute cellular necrosis did not occur until 48 h with at least 200 µM DCVCS, indicating that this is a high-dose, late response. The extent of necrosis was similar to that with DCVC. In contrast, apoptosis occurred as early as 1 h with as little as 10 µM DCVCS and the extent of apoptosis was much less than that with DCVC. Mitochondrial function was maintained with DCVCS concentrations up to 100 µM, consistent with hPT cells only being competent to undergo apoptosis at early time points and relatively low concentrations. Marked depletion (>50%) of cellular GSH content was only observed with 500 µM DCVCS. These results, combined with previous studies showing protection from DCVC-induced necrosis and apoptosis by the FMO inhibitor methimazole, suggest that formation of DCVCS plays a significant role in trichloroethylene-induced renal cellular injury in hPT cells.

Address correspondence to: Dr. Lawrence H. Lash, Department of Pharmacology, Wayne State University School of Medicine, 540 East Canfield Ave., Detroit, MI 48201. E-mail: l.h.lash{at}wayne.edu

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