Vol. 304, Issue 3, 1299-1306, March 2003

Up-Regulation of Regulator of G Protein Signaling 4 Expression in a Model of Neuropathic Pain and Insensitivity to Morphine

Martine Garnier , Paola F. Zaratin , Giovanna Ficalora , Maurizio Valente , Laura Fontanella , Man-Hee Rhee, Kendall J. Blumer and Mark A. Scheideler

Department of Neurobiology Research, GlaxoSmithKline Pharmaceuticals, Milan, Italy (M.G., P.F.Z., G.F., M.V., L.F., M.A.S.); Neurology Centre of Excellence for Drug Discovery, New Frontiers Science Park, Harlow, Essex, United Kingdom (M.G., P.F.Z., G.F., M.V., L.F., M.A.S.); and Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri (M.-H.R., K.J.B.)

We hypothesized that the up-regulated expression of one or more members of the regulator of G protein signaling (RGS) family can cause an attenuation of signaling via Gi/Go-coupled opioid receptors, and thereby play a role in the development of hyperalgesia and accompanying insensitivity to morphine observed in animal models of neuropathic pain. Accordingly, we examined the mRNA expression of several RGS genes in a rat model of chronic neuropathic pain induced by partial ligation of the sciatic nerve. During the development of hyperalgesia, RGS4 was the only isoform examined whose mRNA levels increased significantly (up to 230%) in the lumbar spinal cord. In situ hybridization studies confirmed that RGS4 is present in the dorsal horn of the spinal cord where µ-opioid receptors (MORs) are also expressed. Overexpression of RGS4 in human embryonic kidney 293 cells stably expressing µ-opioid receptors predictably attenuated opioid agonist-induced inhibition of adenylyl cyclase. This inhibitory effect was overcome partially at high agonist concentrations, supporting the view that morphine insensitivity is promoted by RGS4 overexpression. These studies provide evidence that the up-regulation of RGS4 expression may contribute to changes in pain signal processing that lead to the development of hyperalgesia, and further affect its modulation by morphine.