JPET Assistant Professor of Medicine (Clinician-Educator)

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kitamura, A.
Right arrow Articles by Narahashi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kitamura, A.
Right arrow Articles by Narahashi, T.

Vol. 304, Issue 1, 162-171, January 2003

Effects of Halothane and Propofol on Excitatory and Inhibitory Synaptic Transmission in Rat Cortical Neurons

Akira Kitamura, William Marszalec, Jay Z. Yeh and Toshio Narahashi

Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, Illinois

General anesthetics are thought to act on both excitatory and inhibitory neuronal pathways at both post- and presynaptic sites. However, the literature in these regards is somewhat controversial. The aim of the present study was to reassess the relative importance of the various anesthetic actions using a common preparation. Rat cortical neurons in primary culture were used to record spontaneous miniature postsynaptic currents by the whole-cell patch-clamp technique. Halothane at clinically relevant concentrations prolonged the decay phase of spontaneous miniature inhibitory postsynaptic currents (mIPSCs) recorded in the presence of tetrodotoxin and at higher concentrations decreased the frequency of mIPSCs. The mIPSC amplitudes underwent little change. Spontaneous action potential-dependent IPSCs recorded in the absence of tetrodotoxin were similarly affected by halothane. Halothane also decreased the frequency of spontaneous miniature non-N-methyl-D-aspartate (NMDA) excitatory postsynaptic currents (mEPSCs) as well as spontaneous action potential-dependent NMDA EPSCs and non-NMDA EPSCs without affecting their decay phase. The halothane effect on mIPSC and mEPSC frequency was dependent on the external calcium concentration. In contrast to halothane, the only effect of propofol was the prolongation of the decay phase of mIPSCs and IPSCs. The prolongation of mIPSCs and IPSCs by halothane and propofol coupled with the ineffectiveness on mEPSCs and EPSCs suggests a selective postsynaptic modulation of GABAA receptors. The additional calcium-dependent inhibition of mIPSC and mEPSC frequency by halothane (but not propofol) suggests a more general mechanism by this anesthetic on presynaptic transmitter release.

0022-3565/03/3041-0162$07.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 2003 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 Br J AnaesthHome page
D. Turina, V. M. Loitto, K. Bjornstrom, T. Sundqvist, and C. Eintrei
Propofol causes neurite retraction in neurones
Br. J. Anaesth., September 1, 2008; 101(3): 374 - 379.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. R. Drasbek, K. Hoestgaard-Jensen, and K. Jensen
Modulation of Extrasynaptic THIP Conductances by GABAA-Receptor Modulators in Mouse Neocortex
J Neurophysiol, March 1, 2007; 97(3): 2293 - 2300.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
C.-X. Yang, H. Xu, K.-Q. Zhou, M.-Y. Wang, and T.-L. Xu
Modulation of Gamma-Aminobutyric AcidA Receptor Function by Thiopental in the Rat Spinal Dorsal Horn Neurons.
Anesth. Analg., April 1, 2006; 102(4): 1114 - 1120.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
S. K. Georgiev, A. Wakai, T. Kohno, T. Yamakura, and H. Baba
Actions of Norepinephrine and Isoflurane on Inhibitory Synaptic Transmission in Adult Rat Spinal Cord Substantia Gelatinosa Neurons
Anesth. Analg., January 1, 2006; 102(1): 124 - 128.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S.-W. Ying and P. A. Goldstein
Propofol-Block of SK Channels in Reticular Thalamic Neurons Enhances GABAergic Inhibition in Relay Neurons
J Neurophysiol, April 1, 2005; 93(4): 1935 - 1948.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. Verkhratsky
Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons
Physiol Rev, January 1, 2005; 85(1): 201 - 279.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
A. Kitamura, R. Sato, W. Marszalec, J. Z. Yeh, R. Ogawa, and T. Narahashi
Halothane and Propofol Modulation of {gamma}-Aminobutyric AcidA Receptor Single-Channel Currents
Anesth. Analg., August 1, 2004; 99(2): 409 - 415.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2003 by the American Society for Pharmacology and Experimental Therapeutics.