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Vol. 302, Issue 2, 681-686, August 2002
Division of Infection, Inflammation and Repair, School of Medicine,
University of Southampton, Southampton, United Kingdom (G.F.C., P.B.,
M.K.C.), and Biomedical Sciences, Imperial College, London, United
Kingdom (C.C.M.)
The aim of this study was to investigate the impact of
changes in local blood flow on the recovery of a small, diffusible molecule (sodium fluorescein) from the extravascular tissue space of
the skin, by microdialysis in vivo. Loss and recovery of fluorescein by
linear microdialysis probes (5-kDa molecular mass cutoff, 0.2 mm
diameter) inserted 1 mm apart in pairs, at three sites in the skin of
the volar surface of the forearm of healthy volunteers, was measured
under conditions of basal, reduced (noradrenaline, 0.005 mg/ml), and
increased (glyceryl trinitrate, patch) blood flow. Whereas loss
of tracer from the delivery probe appeared unaffected by changes in
local blood flow, retrieval of fluorescein by the second probe was
directly related to blood flux, measured using scanning laser Doppler
imaging. Steady-state recovery at vasoconstricted sites was 4.0 ± 0.7 µg · ml
1 compared with 1.8 ± 0.7 µg · ml1 at control sites (p < 0.001). Local vasodilatation reduced the retrieval of fluorescein by
~50% to give a steady-state concentration of fluorescein in the
dialysate at 40 to 50 min after the start of perfusion of 0.9 ± 0.3 µg · ml1 (p = 0.05).
These studies in the skin are consistent with microdialysis theory.
They suggest that clearance of solute by the blood will have a
significant impact on microdialysis probe recovery and that, in the
skin, the magnitude of this clearance is directly related to blood flow.
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