Vol. 302, Issue 2, 560-567, August 2002

Modulation of Neuronal Nicotinic Acetylcholine Receptors by Mercury

Armen Mirzoian and Charles W. Luetje

Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, Florida

Mercuric chloride exerted a biphasic modulatory effect on rat neuronal nicotinic acetylcholine receptors (nAChRs) expressed in Xenopus laevis oocytes as heteromers of the 3 or 4 and 2 or 4 subunits. The degree of modulation was subunit-dependent, with 4-containing receptors displaying greater potentiation and 4-containing receptors displaying greater inhibition. Thus, 44 receptors displayed both robust potentiation and robust inhibition. During prolonged coapplication of HgCl₂, first potentiation then inhibition of the acetylcholine (ACh) response was observed. Upon coapplication of 1 µM HgCl₂, a 2-fold increase in ACh-induced current was achieved in 55 ± 1 s. With continued HgCl₂ application, the ACh response was slowly inhibited until, after 5 min, less than 10% of the initial response remained. By measuring potentiation at its peak and inhibition 5 min after the start of HgCl₂ coapplication, we obtained EC₅₀ and IC₅₀ values of 262 ± 75 and 430 ± 72 nM, respectively. HgCl₂ potentiation was voltage-dependent, increasing at more positive holding potentials. Upon washout of mercury chloride, potentiation reversed with a t_1/2 of 4.6 min. Inhibition reversed more slowly, with less than half the initial response recovered after 15 min of wash. Although free cysteine residues are common targets for mercury, elimination of all free cysteines located in the extracellular domains of the 4 and 4 subunits did not alter the effects of mercuric chloride. Potentiation and inhibition of neuronal nAChRs may occur through action at a transmembrane or cytoplasmic location after passive diffusion of mercuric chloride across the plasma membrane.