Vol. 300, Issue 2, 668-672, February 2002

Tenidap, an Anti-Inflammatory Agent, Inhibits DNA and Collagen Syntheses, Depresses Cell Proliferation, and Lowers Intracellular pH in Cultured Human Gingival Fibroblasts

Hiroko Matsumoto and Akira Fujii

Department of Pharmacology, Nihon University School of Dentistry at Matsudo, Matsudo, Japan

The effect of tenidap [(±)-5-chloro-2,3-dihydro-3-(hydroxy-2-thienylmethylene)-2-oxo-1H-indole-1-carboxamide], a new anti-inflammatory agent, was investigated on DNA synthesis by means of [³H]thymidine incorporation, collagen synthesis by means of [³H]proline incorporation, cell proliferation, and intracellular pH in nicardipine-reactive human gingival fibroblasts. Tenidap significantly inhibited [³H]thymidine incorporation at concentrations greater than 20 µM on the 4th and 8th day of treatment. Tenidap also significantly inhibited [³H]proline incorporation at a concentration greater than 50 µM on the 4th day and at more than 20 µM on the 8th day of treatment. The presence of 1 µM nifedipine or 1 µM nicardipine did not alter the depressing effect of tenidap. Tenidap (20 µM) also lowered intracellular pH. These results suggest that tenidap might be effective for the prevention of gingival overgrowth caused by calcium channel blockers.