Vol. 300, Issue 2, 638-646, February 2002

Methyl Jasmonate-Induced Stimulation of Sarcoplasmic Reticulum Ca²⁺-ATPase Affects Contractile Responses in Rat Slow-Twitch Skeletal Muscle

Wissam H. Joumaa, Aicha Bouhlel, William Même and Claude Léoty

Laboratoire de Physiologie Générale, Centre National de la Recherche Scientifique UMR 6018, Université de Nantes, Faculté des Sciences et des Techniques, Nantes, France

The purpose of this study was to determine whether methyl jasmonate, a stimulator of Ca²⁺-adenosine triphosphatase (ATPase) activity of the purified ATPase from fast-twitch skeletal muscle, could affect contractile responses in small bundles of rat isolated slow-twitch (soleus) fibers. In saponin-skinned fibers, sarcoplasmic reticulum (SR) Ca²⁺ loading was performed in pCa 7.0 solution. The amount of Ca²⁺ taken up was monitored by use of the amplitude of contraction following application of 10 mM caffeine. Results indicate that the increased loading rate in the presence of methyl jasmonate is likely due to stimulation of the SR Ca²⁺-ATPase. In Triton-skinned fibers, the myofibrillar Ca²⁺ sensitivity was not changed by methyl jasmonate (50-200 µM). In intact fibers, the amplitude and the time constant of relaxation of twitch and potassium contracture were reversibly reduced after 2 min of application of methyl jasmonate at a concentration of up to 125 µM. At higher concentrations (>150 µM), effects were not reversible. In the presence of methyl jasmonate (100 µM), the relationship between the amplitude of potassium contractures and the membrane potential shifted to more positive potentials, whereas the steady-state inactivation curve was unchanged. These observations suggest that methyl jasmonate has no effect on voltage sensors. Taken together, our results show that methyl jasmonate is a potent, reversible, and specific stimulator of the SR Ca²⁺ pump in slow-twitch skeletal muscle and is an extremely valuable pharmacological tool for improving relaxation and studying calcium-signaling questions.