![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 300, Issue 1, 188-194, January 2002
New York Medical College, Department of Pharmacology, Valhalla, New
York (A.H., M.L.S., A.N., N.G.A.); Jagiellonian University, Medical
College, Department of Pharmacology, Cracow, Poland (R.O., R.G.);
Robert Wood Johnson Medical School, New Brunswick, New Jersey (E.L.);
and The Rockefeller University, New York, New York (A.K.)
Heme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves
heme to form biliverdin, with the release of iron and carbon monoxide
(CO). HO not only controls the availability of heme for the synthesis
of heme proteins but also is responsible for the generation of CO,
which binds to the heme moiety of heme proteins thus affecting their
enzymatic activity. Cyclooxygenase (COX) is a heme protein that
catalyzes the conversion of arachidonic acid to prostaglandin
H2, the precursor of prostanoids that participate in the
regulation of vascular function. The goal of the present study was to
determine whether the heme-HO system regulates COX enzyme expression
and activity in vascular endothelial cells. Endothelial cells stably
transfected with the human HO-1 gene exhibited a severalfold increase
in human HO-1 mRNA levels, which was accompanied by an increase in HO
activity and a marked decrease in prostaglandin (PG) E2 and
6-keto-PGF1
levels. Exposure of cells to
CoCl2, an inducer of HO-1 gene expression, resulted in
increases in HO-1 protein levels and HO activity. The increase in HO
activity was associated with a subsequent decrease in COX activity,
which returned to normal levels following normalization of HO activity.
The addition of heme resulted in an increase in COX activity with an
increase in PGE2 and 6-keto-PGF1 levels. The
degree of HO-1 expression and, consequently, the level of cellular
heme, were directly related to COX activity. These results demonstrate
that the heme-HO system can function as a cellular regulator of the
expression of vascular COX, thus influencing the generation of
prostanoids, PGE2 and PGI2, known to play a role in vascular homeostasis.
This article has been cited by other articles:
|
|
 |
|
  F. T. Botros, M. C. Prieto-Carrasquero, V. L. Martin, and L. G. Navar Heme oxygenase induction attenuates afferent arteriolar autoregulatory responses Am J Physiol Renal Physiol, October 1, 2008; 295(4): F904 - F911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Patil, L. Bellner, G. Cullaro, K. H. Gotlinger, M. W. Dunn, and M. L. Schwartzman Heme Oxygenase-1 Induction Attenuates Corneal Inflammation and Accelerates Wound Healing after Epithelial Injury Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3379 - 3386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Stec, H. A. Drummond, and T. Vera Role of Carbon Monoxide in Blood Pressure Regulation Hypertension, March 1, 2008; 51(3): 597 - 604. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Kronke, A. Kadl, E. Ikonomu, S. Bluml, A. Furnkranz, I. J. Sarembock, V. N. Bochkov, M. Exner, B. R. Binder, and N. Leitinger Expression of Heme Oxygenase-1 in Human Vascular Cells Is Regulated by Peroxisome Proliferator-Activated Receptors Arterioscler. Thromb. Vasc. Biol., June 1, 2007; 27(6): 1276 - 1282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Villanueva, C. Cespedes, A. A. Gonzalez, C. P. Vio, and V. Velarde Effect of ischemic acute renal damage on the expression of COX-2 and oxidative stress-related elements in rat kidney Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1364 - F1371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Li, F. Yi, E. A. dos Santos, D. K. Donley, and P.-L. Li Role of Renal Medullary Heme Oxygenase in the Regulation of Pressure Natriuresis and Arterial Blood Pressure Hypertension, January 1, 2007; 49(1): 148 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Stocker and M. A. Perrella Heme Oxygenase-1: A Novel Drug Target for Atherosclerotic Diseases? Circulation, November 14, 2006; 114(20): 2178 - 2189. [Full Text] [PDF]
|
|
|
|
 |
|
 F. Seta, L. Bellner, R. Rezzani, R. F. Regan, M. W. Dunn, N. G. Abraham, K. Gronert, and M. Laniado-Schwartzman Heme Oxygenase-2 Is a Critical Determinant for Execution of an Acute Inflammatory and Reparative Response Am. J. Pathol., November 1, 2006; 169(5): 1612 - 1623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Rydkina, A. Sahni, R. B. Baggs, D. J. Silverman, and S. K. Sahni Infection of Human Endothelial Cells with Spotted Fever Group Rickettsiae Stimulates Cyclooxygenase 2 Expression and Release of Vasoactive Prostaglandins Infect. Immun., September 1, 2006; 74(9): 5067 - 5074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Goodman, P. N. Chander, R. Rezzani, M. L. Schwartzman, R. F. Regan, L. Rodella, S. Turkseven, E. A. Lianos, P. A. Dennery, and N. G. Abraham Heme Oxygenase-2 Deficiency Contributes to Diabetes-Mediated Increase in Superoxide Anion and Renal Dysfunction J. Am. Soc. Nephrol., April 1, 2006; 17(4): 1073 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ishikawa, M. Kajimura, T. Adachi, K. Maruyama, N. Makino, N. Goda, T. Yamaguchi, E. Sekizuka, and M. Suematsu Carbon Monoxide From Heme Oxygenase-2 Is a Tonic Regulator Against NO-Dependent Vasodilatation in the Adult Rat Cerebral Microcirculation Circ. Res., December 9, 2005; 97(12): e104 - e114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hill, V. Pereira, C. Chauveau, R. Zagani, S. Remy, L. Tesson, D. Mazal, L. Ubillos, R. Brion, K. Ashgar, et al. Heme oxygenase-1 inhibits rat and human breast cancer cell proliferation: mutual cross inhibition with indoleamine 2,3-dioxygenase FASEB J, December 1, 2005; 19(14): 1957 - 1968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhou, F. Lu, C. Latham, D. S. Zander, and G. A. Visner Heme Oxygenase-1 Expression in Human Lungs with Cystic Fibrosis and Cytoprotective Effects against Pseudomonas Aeruginosa In Vitro Am. J. Respir. Crit. Care Med., September 15, 2004; 170(6): 633 - 640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Taille, J. El-Benna, S. Lanone, M.-C. Dang, E. Ogier-Denis, M. Aubier, and J. Boczkowski Induction of Heme Oxygenase-1 Inhibits NAD(P)H Oxidase Activity by Down-regulating Cytochrome b558 Expression via the Reduction of Heme Availability J. Biol. Chem., July 2, 2004; 279(27): 28681 - 28688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yang, S. Quan, A. Nasjletti, M. Laniado-Schwartzman, and N. G. Abraham Heme Oxygenase-1 Gene Expression Modulates Angiotensin II-Induced Increase in Blood Pressure Hypertension, June 1, 2004; 43(6): 1221 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Vicente, M. I. Guillen, A. Habib, and M. J. Alcaraz Beneficial Effects of Heme Oxygenase-1 Up-Regulation in the Development of Experimental Inflammation Induced by Zymosan J. Pharmacol. Exp. Ther., December 1, 2003; 307(3): 1030 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Thengchaisri and L. Kuo Hydrogen peroxide induces endothelium-dependent and -independent coronary arteriolar dilation: role of cyclooxygenase and potassium channels Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2255 - H2263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Demasi, L. G. Cleland, R. J. Cook-Johnson, G. E. Caughey, and M. J. James Effects of Hypoxia on Monocyte Inflammatory Mediator Production: DISSOCIATION BETWEEN CHANGES IN CYCLOOXYGENASE-2 EXPRESSION AND EICOSANOID SYNTHESIS J. Biol. Chem., October 3, 2003; 278(40): 38607 - 38616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rodriguez, R. Kemp, M. Balazy, and A. Nasjletti Effects of Exogenous Heme on Renal Function: Role of Heme Oxygenase and Cyclooxygenase Hypertension, October 1, 2003; 42(4): 680 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. D. T. G. Wagener, H.-D. Volk, D. Willis, N. G. Abraham, M. P. Soares, G. J. Adema, and C. G. Figdor Different Faces of the Heme-Heme Oxygenase System in Inflammation Pharmacol. Rev., September 1, 2003; 55(3): 551 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rodriguez, F. Zhang, S. Dinocca, and A. Nasjletti Nitric oxide synthesis influences the renal vascular response to heme oxygenase inhibition Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1255 - F1262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Malaguarnera, S. Quan, M. R. Pilastro, N. G. Abraham, and A. Kappas Diminished Heme Oxygenase Potentiates Cell Death: Pyrrolidinedithiocarbamate Mediates Oxidative Stress Experimental Biology and Medicine, May 1, 2003; 228(5): 459 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Vicente, M. I. Guillin, and M. J. Alcaraz Participation of Heme Oxygenase-1 in a Model of Acute Inflammation Experimental Biology and Medicine, May 1, 2003; 228(5): 514 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Kiemer, N. Bildner, N. C. Weber, and A. M. Vollmar Characterization of Heme Oxygenase 1 (Heat Shock Protein 32) Induction by Atrial Natriuretic Peptide in Human Endothelial Cells Endocrinology, March 1, 2003; 144(3): 802 - 812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Li Volti, F. Seta, M. L. Schwartzman, A. Nasjletti, and N. G. Abraham Heme Oxygenase Attenuates Angiotensin II-Mediated Increase in Cyclooxygenase-2 Activity in Human Femoral Endothelial Cells Hypertension, March 1, 2003; 41(3): 715 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. T. Botros, M. Laniado-Schwartzman, and N. G. Abraham Regulation of Cyclooxygenase- and Cytochrome P450-Derived Eicosanoids by Heme Oxygenase in the Rat Kidney Hypertension, February 1, 2002; 39(2): 639 - 644. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
