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Vol. 299, Issue 3, 934-938, December 2001

Increase in Neurokinin B Expression and in Tachykinin NK3 Receptor-Mediated Response and Expression in the Rat Uterus with Age

Cristina G. Cintado1 , Francisco M. Pinto, Philippe Devillier, Angel Merida and M. Luz Candenas

Institutes of Chemical Research (C.G.C., F.M.P., M.L.C.) and Biochemistry (A.M.), Scientific Research Center Isla de La Cartuja, Sevilla, Spain and Laboratoire de Pharmacologie-Toxicologie (P.D.), Centre Hospitalier Universitaire, Reims, France

We analyzed tachykinin NK3 receptor (NK3R) gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) in uteri from young (3-month-old) and old (30-month-old) rats. In addition, we characterized the expression of the preprotachykinin-B (TAC-3) gene, which encodes neurokinin B (NKB), the preferred endogenous agonist of NK3R. Compared with young rats, NK3R messenger RNA (mRNA) levels were about 45-fold higher in uteri from old animals. TAC-3 mRNA was expressed in the rat uterus, and its levels were about 2.5-fold higher in old than in young rats. The contractile effect of the selective tachykinin NK3R agonist [MePhe7]-NKB in uteri from young and old animals was investigated by using conventional organ bath technique. A marked correlation was observed between the magnitude of the contraction elicited by [MePhe7]-NKB and the level of expression determined by RT-PCR for the NK3R. These observations are consistent with a role for the NKB/NK3R ligand-receptor pair in regulating uterine functions and support the existence of a link between estrogen and the NK3R/NKB activation pathway.

1 Recipient of a fellowship from the Ministry of Science and Technology (Spain).

0022-3565/01/2993-0934$03.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 2001 by The American Society for Pharmacology and Experimental Therapeutics


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