JPET Assistant Professor of Medicine (Clinician-Educator)

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hernandez-Benito, M. J.
Right arrow Articles by Mubagwa, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hernandez-Benito, M. J.
Right arrow Articles by Mubagwa, K.

Vol. 298, Issue 2, 598-606, August 2001

Suppression of Transient Outward Potassium Currents in Mouse Ventricular Myocytes by Imidazole Antimycotics and by Glybenclamide

M. J. Hernandez-Benito , R. Macianskiene , K. R. Sipido, W. Flameng and K. Mubagwa

Laboratory of Cardiac Cellular Research, Centre for Experimental Surgery and Anaesthesiology (M.J.H.-B., R.M., W.F., K.M.), and Laboratory of Experimental Cardiology (K.R.S.), University of Leuven, Leuven, Belgium

The whole-cell patch-clamp technique was used in adult mouse ventricular myocytes at 22°C to study the transient outward current (Ito) and its sensitivity to the antimycotics miconazole and clotrimazole, as well as to glybenclamide. Ito elicited by depolarizing steps from a holding potential of -80 mV consisted of a fast inactivating component and a slowly inactivating component. In the presence of miconazole (IC50 of approx 8 µM) or clotrimazole, Ito peak amplitude was reduced and its inactivation accelerated, due to a selective suppression of the slow component, without an effect on the fast component or on the noninactivating current. The effect did not reverse upon washout, was not induced by intracellular drug application, and occurred without a change of the steady-state inactivation. In the presence of glybenclamide Ito peak amplitude was reduced and its inactivation accelerated. In contrast to the antimycotics, glybenclamide suppressed both the fast and the slow components (IC50 of approx 50 µM), its effect was reversible, and was associated with a negative shift of the steady-state inactivation. These data demonstrate a pharmacological separation of Ito components using antimycotic drugs but not glybenclamide.

0022-3565/01/2982-0598$03.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 2001 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 J. Physiol.Home page
K. Fukuzaki, T. Sato, T. Miki, S. Seino, and H. Nakaya
Role of sarcolemmal ATP-sensitive K+ channels in the regulation of sinoatrial node automaticity: an evaluation using Kir6.2-deficient mice
J. Physiol., June 1, 2008; 586(11): 2767 - 2778.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. W. deHart, T. Jin, D. E. McCloskey, A. E. Pegg, and D. Sheppard
The {alpha}9{beta}1 integrin enhances cell migration by polyamine-mediated modulation of an inward-rectifier potassium channel
PNAS, May 20, 2008; 105(20): 7188 - 7193.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S.-Y. Lee and C. O. Lee
Inhibition of Na+-K+ Pump and L-Type Ca2+ Channel by Glibenclamide in Guinea Pig Ventricular Myocytes
J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 61 - 68.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
E. Bussemaker, R. Popp, B. Fisslthaler, C. M. Larson, I. Fleming, R. Busse, and R. P. Brandes
Aged Spontaneously Hypertensive Rats Exhibit a Selective Loss of EDHF-Mediated Relaxation in the Renal Artery
Hypertension, October 1, 2003; 42(4): 562 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Macianskiene, F. Moccia, K. R. Sipido, W. Flameng, and K. Mubagwa
Channels involved in transient currents unmasked by removal of extracellular calcium in cardiac cells
Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1879 - H1888.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2001 by the American Society for Pharmacology and Experimental Therapeutics.