JPET Celsis microsomes equal better data

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhong, H.
Right arrow Articles by Blackburn, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhong, H.
Right arrow Articles by Blackburn, M. R.

Vol. 298, Issue 2, 433-440, August 2001

Adenosine-Mediated Mast Cell Degranulation in Adenosine Deaminase-Deficient Mice

Hongyan Zhong, Janci L. Chunn, Jonathan B. Volmer, John R. Fozard and Michael R. Blackburn

Department of Biochemistry and Molecular Biology, The University of Texas-Houston Medical School, Houston, Texas (H.Z., J.L.C., J.B.V., M.R.B.); and Research Department, Novartis Pharma AG, Basel, Switzerland (J.R.F.)

Adenosine is a signaling nucleoside that has been suggested to play a role in asthma in part through its ability to influence mediator release from mast cells. Adenosine levels are elevated in the lungs of asthmatics, further implicating this molecule in the regulation of lung inflammation and suggesting that animal models exhibiting endogenous increases in adenosine will be useful for the analysis of adenosine function. Adenosine deaminase (ADA) is a purine catabolic enzyme responsible for regulating the levels of adenosine in tissues and cells. ADA-deficient mice develop lung inflammation and damage reminiscent of that seen in asthma in association with elevated adenosine levels. In the current study, we investigated the status of mast cells in ADA-deficient lungs. ADA-deficient mice exhibited extensive lung mast cell degranulation concurrent with elevated adenosine levels. ADA enzyme therapy prevented the accumulation of lung adenosine as well as mast cell degranulation, suggesting that this process was dependent on elevated lung adenosine levels. Consistent with this, treatment of ADA-deficient mice with broad spectrum adenosine receptor antagonists attenuated degranulation by 30 to 40%, supporting the involvement of adenosine receptor signaling. Moreover, these studies demonstrate the ability of endogenously generated adenosine to influence lung mast cell degranulation in a receptor-mediated manner and establish ADA-deficient mice as a model system to investigate the specific adenosine receptor responses involved in the degranulation of lung mast cells.

0022-3565/01/2982-0433$03.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 2001 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
S. Ryzhov, A. E. Goldstein, I. Biaggioni, and I. Feoktistov
Cross-Talk between Gs- and Gq-Coupled Pathways in Regulation of Interleukin-4 by A2B Adenosine Receptors in Human Mast Cells
Mol. Pharmacol., August 1, 2006; 70(2): 727 - 735.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
P. J. Oldenburg and S. J. Mustafa
Involvement of Mast Cells in Adenosine-Mediated Bronchoconstriction and Inflammation in an Allergic Mouse Model
J. Pharmacol. Exp. Ther., April 1, 2005; 313(1): 319 - 324.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. W. J. Young, J. G. Molina, D. Dimina, H. Zhong, M. Jacobson, L.-N. L. Chan, T.-S. Chan, J. J. Lee, and M. R. Blackburn
A3 Adenosine Receptor Signaling Contributes to Airway Inflammation and Mucus Production in Adenosine Deaminase-Deficient Mice
J. Immunol., July 15, 2004; 173(2): 1380 - 1389.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Ryzhov, A. E. Goldstein, A. Matafonov, D. Zeng, I. Biaggioni, and I. Feoktistov
Adenosine-Activated Mast Cells Induce IgE Synthesis by B Lymphocytes: An A2B-Mediated Process Involving Th2 Cytokines IL-4 and IL-13 with Implications for Asthma
J. Immunol., June 15, 2004; 172(12): 7726 - 7733.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. K. Banerjee, H. W. J. Young, A. Barczak, D. J. Erle, and M. R. Blackburn
Abnormal Alveolar Development Associated with Elevated Adenine Nucleosides
Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 38 - 50.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. Zhong, L. Belardinelli, T. Maa, I. Feoktistov, I. Biaggioni, and D. Zeng
A2B Adenosine Receptors Increase Cytokine Release by Bronchial Smooth Muscle Cells
Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 118 - 125.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Zhong, S. G. Shlykov, J. G. Molina, B. M. Sanborn, M. A. Jacobson, S. L. Tilley, and M. R. Blackburn
Activation of Murine Lung Mast Cells by the Adenosine A3 Receptor
J. Immunol., July 1, 2003; 171(1): 338 - 345.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Fan, W. Qin, and S. J. Mustafa
Characterization of adenosine receptor(s) involved in adenosine-induced bronchoconstriction in an allergic mouse model
Am J Physiol Lung Cell Mol Physiol, June 1, 2003; 284(6): L1012 - L1019.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2001 by the American Society for Pharmacology and Experimental Therapeutics.