JPET Assistant Professor of Medicine (Clinician-Educator)

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, M.
Right arrow Articles by Burnstock, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, M.
Right arrow Articles by Burnstock, G.

Vol. 296, Issue 3, 1043-1050, March 2001

Coexpression of P2X3 and P2X2 Receptor Subunits in Varying Amounts Generates Heterogeneous Populations of P2X Receptors That Evoke a Spectrum of Agonist Responses Comparable to That Seen in Sensory Neurons

Min Liu, Brian F. King, Philip M. Dunn, Weifang Rong, Andrea Townsend-Nicholson and Geoffrey Burnstock

Autonomic Neuroscience Institute, Royal Free and University College Medical School, Royal Free Campus, Hampstead, London, United Kingdom

Using voltage-clamp procedures on Xenopus oocytes, agonist-evoked ionic currents by P2X receptors resulting from the coexpression of P2X2 and P2X3 subunits were compared against the agonist responses of homomeric P2X2 and P2X3 receptors. With the quantity of P2X3 mRNA kept constant and quantity of P2X2 mRNA progressively increased, expressed P2X receptors changed from a P2X3-like receptor to a P2X2-like receptor. In all cases, however, agonist-evoked responses comprised biphasic (fast and slow) currents---the former showing the properties of P2X3 receptors and latter consistent with the presence of P2X2 and P2X2/3 receptors. Using desensitization procedures, the P2X3-like fast current was selectively removed to allow the slow current to be studied in isolation. P2X2/3 receptors were then characterized by slowly inactivating inward currents that were reproducible within 30 s of washout and whose pharmacological profile [selective agonists, Ap5A > alpha ,beta -methylene ATP beta ,gamma -methylene ATP > UTP; antagonists, TNP-ATP suramin >=  Reactive blue-2 (RB-2)] contrasted with the profile of P2X2 receptors (Ap5A, alpha ,beta -methylene ATP, beta ,gamma -methylene ATP, and UTP inactive; antagonists, RB-2 > TNP-ATP > suramin). Thus, our experiments reveal that coexpression of two P2X subunits, which of themselves can generate functional homomeric receptors, results in a complex population of heterogeneous P2X receptors---in this case P2X2, P2X3, and P2X2/3 receptors. Depending on the relative levels of P2X subunit coexpression, the operational profile of the resultant P2X receptors can change from one phenotype to another. This spectrum may explain the variability of agonist responses in small sensory neurons that also express P2X2 and P2X3 subunits in different amounts.

0022-3565/01/2963-1043$03.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 2001 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
K. Dang, K. Lamb, M. Cohen, K. Bielefeldt, and G. F. Gebhart
Cyclophosphamide-Induced Bladder Inflammation Sensitizes and Enhances P2X Receptor Function in Rat Bladder Sensory Neurons
J Neurophysiol, January 1, 2008; 99(1): 49 - 59.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. D'Arco, R. Giniatullin, M. Simonetti, A. Fabbro, A. Nair, A. Nistri, and E. Fabbretti
Neutralization of Nerve Growth Factor Induces Plasticity of ATP-Sensitive P2X3 Receptors of Nociceptive Trigeminal Ganglion Neurons
J. Neurosci., August 1, 2007; 27(31): 8190 - 8201.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Burnstock
Physiology and Pathophysiology of Purinergic Neurotransmission
Physiol Rev, April 1, 2007; 87(2): 659 - 797.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. Greenwood, D. J. Jagger, L.-C. Huang, N. Hoya, P. R. Thorne, S. S. Wildman, B. F. King, K. Pak, A. F. Ryan, and G. D. Housley
P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea
Development, April 1, 2007; 134(7): 1407 - 1417.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
R. Hausmann, J. Rettinger, Z. Gerevich, S. Meis, M. U. Kassack, P. Illes, G. Lambrecht, and G. Schmalzing
The Suramin Analog 4,4',4'',4'''-(Carbonylbis(imino-5,1,3-benzenetriylbis (carbonylimino)))tetra-kis-benzenesulfonic Acid (NF110) Potently Blocks P2X3 Receptors: Subtype Selectivity Is Determined by Location of Sulfonic Acid Groups
Mol. Pharmacol., June 1, 2006; 69(6): 2058 - 2067.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. A. P. Chaves, E. P. Pontelli, and W. A. Varanda
P2X receptors in mouse Leydig cells
Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1009 - C1017.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Dang, K. Bielfeldt, K. Lamb, and G. F. Gebhart
Gastric Ulcers Evoke Hyperexcitability and Enhance P2X Receptor Function in Rat Gastric Sensory Neurons
J Neurophysiol, June 1, 2005; 93(6): 3112 - 3119.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Tsuzuki, A. Ase, P. Seguela, T. Nakatsuka, C.-Y. Wang, J.-X. She, and J. G. Gu
TNP-ATP-Resistant P2X Ionic Current on the Central Terminals and Somata of Rat Primary Sensory Neurons
J Neurophysiol, June 1, 2003; 89(6): 3235 - 3242.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Li and L. I. Sinoway
ATP stimulates chemically sensitive and sensitizes mechanically sensitive afferents
Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2636 - H2643.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. S. Wildman, S. G. Brown, M. Rahman, C. A. Noel, L. Churchill, G. Burnstock, R. J. Unwin, and B. F. King
Sensitization by Extracellular Ca2+ of Rat P2X5 Receptor and Its Pharmacological Properties Compared with Rat P2X1.
Mol. Pharmacol., October 1, 2002; 62(4): 957 - 966.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
R. A. North
Molecular Physiology of P2X Receptors
Physiol Rev, October 1, 2002; 82(4): 1013 - 1067.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. G. Brown, A. Townsend-Nicholson, K. A. Jacobson, G. Burnstock, and B. F. King
Heteromultimeric P2X1/2 Receptors Show a Novel Sensitivity to Extracellular pH
J. Pharmacol. Exp. Ther., February 1, 2002; 300(2): 673 - 680.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Prasad, I. M Fearon, M. Zhang, M. Laing, C. Vollmer, and C. A Nurse
Expression of P2X2 and P2X3 receptor subunits in rat carotid body afferent neurones: role in chemosensory signalling
J. Physiol., December 15, 2001; 537(3): 667 - 677.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
B. A. Chizh and P. Illes
P2X Receptors and Nociception
Pharmacol. Rev., December 1, 2001; 53(4): 553 - 568.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. Sokolova, A. Nistri, and R. Giniatullin
Negative Cross Talk between Anionic GABAA and Cationic P2X Ionotropic Receptors of Rat Dorsal Root Ganglion Neurons
J. Neurosci., July 15, 2001; 21(14): 4958 - 4968.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2001 by the American Society for Pharmacology and Experimental Therapeutics.