JPET Assistant Professor of Medicine (Clinician-Educator)

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gurujeyalakshmi, G.
Right arrow Articles by Giri, S. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gurujeyalakshmi, G.
Right arrow Articles by Giri, S. N.

Vol. 293, Issue 1, 82-90, April 2000

Taurine and Niacin Block Lung Injury and Fibrosis by Down-Regulating Bleomycin-Induced Activation of Transcription Nuclear Factor-kappa B in Mice1,2

Gopalakrishnan Gurujeyalakshmi, Yingjin Wang and Shri N. Giri

Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California

The effects of taurine (T) and niacin (N) on bleomycin (BL)-induced increased production of tumor necrosis factor-alpha (TNF-alpha ), interleukin (IL)-1alpha , IL-6, and transforming growth factor-beta (TGF-beta ) levels in the bronchoalveolar lavage fluid (BALF), and increased collagen content and nuclear factor-kappa B (NF-kappa B) activation in the lungs were investigated in mice. The mice were intratracheally instilled with saline (SA) or BL (0.1 U/mouse/50 µl) under ketamine and xylazine anesthesia. They had ad libitum access to diet containing 2.5% niacin (w/w) or the same control diet (CD) and water with and without taurine (1%) 3 days before intratracheal instillation and throughout the study. The mice were sacrificed at different times for collecting BALF and lungs, which were appropriately processed for various measurements. Treatment with taurine and niacin attenuated the BL-induced increases in proinflammatory cytokines such as IL-1alpha , TNF-alpha , IL-6, and TGF-beta in BALF and lung hydroxyproline content of the mice in BL + TN groups. Reverse transcription-polymerase chain reaction analysis of total RNA from whole lung was performed to assess the induction of TNF-alpha and IL-1 mRNAs as markers of NF-kappa B activation. The NF-kappa B DNA-binding activity in whole-lung extract was evaluated by electrophoretic mobility shift assay. This revealed a progressive increase in NF-kappa B activation and IkBalpha depletion in lungs from mice in BL + CD groups from day 1 through day 21 compared with the corresponding SA + CD control groups. Treatment with taurine and niacin generally inhibited the BL-induced increases in the nuclear localization of NF-kappa B and preserved Ikappa Balpha protein in BL + TN groups. This may be one of the mechanisms for the antifibrotic effect of taurine and niacin.

1 This research was supported by National Heart, Lung, and Blood Institute Grant R01-56262.

2 A preliminary report of this work was presented in part at the International Taurine Symposium; Certosa di Pontignano, Siena, Italy, August 4-8, 1999.

0022-3565/00/2931-0082$03.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 2000 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
H.-J. Lehmler and P. M. Bummer
Interaction of a partially fluorinated long-chain nicotinate with dipalmitoylphosphatidylcholine
J. Lipid Res., November 1, 2005; 46(11): 2415 - 2422.
[Abstract] [Full Text] [PDF]

Home page
 Mult SclerHome page
J. E Walker, S. N Giri, and S. B Margolin
A double-blind, randomized, controlled study of oral pirfenidone for treatment of secondary progressive multiple sclerosis
Multiple Sclerosis, April 1, 2005; 11(2): 149 - 158.
[Abstract] [PDF]

Home page
 J. Lipid Res.Home page
H.-J. Lehmler, A. Fortis-Santiago, D. Nauduri, and P. M. Bummer
Interaction of long-chain nicotinates with dipalmitoylphosphatidylcholine
J. Lipid Res., March 1, 2005; 46(3): 535 - 546.
[Abstract] [Full Text] [PDF]

Home page
 CMAJHome page
N. Khalil and R. O'Connor
Idiopathic pulmonary fibrosis: current understanding of the pathogenesis and the status of treatment
Can. Med. Assoc. J., July 20, 2004; 171(2): 153 - 160.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
S. T. Sonis
THE BIOLOGIC ROLE FOR NUCLEAR FACTOR-KAPPAB IN DISEASE AND ITS POTENTIAL INVOLVEMENT IN MUCOSAL INJURY ASSOCIATED WITH ANTI-NEOPLASTIC THERAPY
Critical Reviews in Oral Biology & Medicine, September 1, 2002; 13(5): 380 - 389.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
A Serrano-Mollar, D Closa, J Cortijo, E J Morcillo, N Prats, M Gironella, J Panes, J Rosello-Catafau, and O Bulbena
P-selectin upregulation in bleomycin induced lung injury in rats: effect of N-acetyl-L-cysteine
Thorax, July 1, 2002; 57(7): 629 - 634.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. G. Luzina, S. P. Atamas, R. Wise, F. M. Wigley, H. Q. Xiao, and B. White
Gene Expression in Bronchoalveolar Lavage Cells from Scleroderma Patients
Am. J. Respir. Cell Mol. Biol., May 1, 2002; 26(5): 549 - 557.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
H. Miyakawa, K. Sato, T. Shinbori, T. Okamoto, Y. Gushima, M. Fujiki, and M. Suga
Effects of inducible nitric oxide synthase and xanthine oxidase inhibitors on SEB-induced interstitial pneumonia in mice
Eur. Respir. J., March 1, 2002; 19(3): 447 - 457.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Barua, Y. Liu, and M. R. Quinn
Taurine Chloramine Inhibits Inducible Nitric Oxide Synthase and TNF-{alpha} Gene Expression in Activated Alveolar Macrophages: Decreased NF-{kappa}B Activation and I{kappa}B Kinase Activity
J. Immunol., August 15, 2001; 167(4): 2275 - 2281.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
J. Cortijo, M. Cerda-Nicolas, A. Serrano, G. Bioque, J.M. Estrela, F. Santangelo, A. Esteras, A. Llombart-Bosch, and E.J. Morcillo
Attenuation by oral N-acetylcysteine of bleomycin-induced lung injury in rats
Eur. Respir. J., June 1, 2001; 17(6): 1228 - 1235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. V. Kalinichenko, L. Lim, B. Shin, and R. H. Costa
Differential expression of forkhead box transcription factors following butylated hydroxytoluene lung injury
Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L695 - L704.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. A. Ortiz, H. C. Champion, J. A. Lasky, F. Gambelli, E. Gozal, G. W. Hoyle, M. B. Beasley, A. L. Hyman, M. Friedman, and P. J. Kadowitz
Enalapril protects mice from pulmonary hypertension by inhibiting TNF-mediated activation of NF-kappa B and AP-1
Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1209 - L1221.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2000 by the American Society for Pharmacology and Experimental Therapeutics.