JPET Assistant Professor of Medicine (Clinician-Educator)

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tan, J.
Right arrow Articles by Robinson, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tan, J.
Right arrow Articles by Robinson, M. B.

Vol. 289, Issue 3, 1600-1610, June 1999

Expression of the GLT-1 Subtype of Na+-Dependent Glutamate Transporter: Pharmacological Characterization and Lack of Regulation by Protein Kinase C1

Jue Tan, Olga Zelenaia, Dana Correale, Jeffrey D. Rothstein and Michael B. Robinson

Departments of Pediatrics and Pharmacology, Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, Pennsylvania (J.T., O.Z., D.C., M.B.R) and Department of Neurology, The Johns Hopkins University, Baltimore, Maryland (J.D.R.)

Several subtypes of Na+-dependent glutamate transporters have been pharmacologically differentiated in brain tissues. Five distinct cDNA clones that express Na+-dependent glutamate transport activity have been isolated. One goal of the current study was to compare the pharmacological properties of the rat GLT-1 subtype of transporter to those identified previously using rat brain tissues. To accomplish this goal, GLT-1 was stably transfected into two different cell lines that express low levels of endogenous transport activity (MCB and L-M (TK-)). Several clones stably transfected with GLT-1 were isolated. In each cell line, Na+-dependent glutamate transport activity was saturable with similar Km values (19 and 37 µM). The pharmacological properties of GLT-1-mediated transport in these cell lines paralleled those observed for the predominant pharmacology observed in cortical crude synaptosomes. These data are consistent with other lines of evidence that suggest that GLT-1 may be sufficient to explain most of the Na+-dependent glutamate transport activity in cortical synaptosomes. Although recent studies using HeLa cells have suggested that GLT-1 can be rapidly up-regulated by activation of protein kinase C (PKC), modulation of PKC or phosphatase activity had no effect on GLT-1-mediated activity in these transfected cell lines. To determine if GLT-1 regulation by PKC is cell-specific, HeLa cells, which endogenously express the EAAC1 subtype of transporter, were stably transfected with GLT-1. Although EAAC1-mediated activity was increased by activation of PKC, we found no evidence for regulation of GLT-1. Despite the present findings, GLT-1 activity may be regulated by PKC under certain conditions.

0022-3565/99/2893-1600$03.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 1999 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
S. Colleoni, A. A. Jensen, E. Landucci, E. Fumagalli, P. Conti, A. Pinto, M. De Amici, D. E. Pellegrini-Giampietro, C. De Micheli, T. Mennini, et al.
Neuroprotective Effects of the Novel Glutamate Transporter Inhibitor (-)-3-Hydroxy-4,5,6,6a-tetrahydro-3aH-pyrrolo[3,4-d]-isoxazole-4-carboxylic Acid, Which Preferentially Inhibits Reverse Transport (Glutamate Release) Compared with Glutamate Reuptake
J. Pharmacol. Exp. Ther., August 1, 2008; 326(2): 646 - 656.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. D. Pita-Almenar, M. S. Collado, C. M. Colbert, and A. Eskin
Different Mechanisms Exist for the Plasticity of Glutamate Reuptake during Early Long-Term Potentiation (LTP) and Late LTP
J. Neurosci., October 11, 2006; 26(41): 10461 - 10471.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Fang, Y. Huang, and Z. Zuo
Enhancement of substrate-gated Cl- currents via rat glutamate transporter EAAT4 by PMA
Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1334 - C1340.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
J. Dunlop, H. B. McIlvain, T. A. Carrick, B. Jow, Q. Lu, D. Kowal, S. Lin, A. Greenfield, C. Grosanu, K. Fan, et al.
Characterization of Novel Aryl-Ether, Biaryl, and Fluorene Aspartic Acid and Diaminopropionic Acid Analogs as Potent Inhibitors of the High-Affinity Glutamate Transporter EAAT2
Mol. Pharmacol., October 1, 2005; 68(4): 974 - 982.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Kalandadze, Y. Wu, K. Fournier, and M. B. Robinson
Identification of Motifs Involved in Endoplasmic Reticulum Retention-Forward Trafficking of the GLT-1 Subtype of Glutamate Transporter
J. Neurosci., June 2, 2004; 24(22): 5183 - 5192.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
W. Chen, V. Mahadomrongkul, U. V. Berger, M. Bassan, T. DeSilva, K. Tanaka, N. Irwin, C. Aoki, and P. A. Rosenberg
The Glutamate Transporter GLT1a Is Expressed in Excitatory Axon Terminals of Mature Hippocampal Neurons
J. Neurosci., February 4, 2004; 24(5): 1136 - 1148.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Interv.Home page
M. I. Gonzalez and M. B. Robinson
Protein KINASE C-Dependent Remodeling of Glutamate Transporter Function
Mol. Interv., February 1, 2004; 4(1): 48 - 58.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kalandadze, Y. Wu, and M. B. Robinson
Protein Kinase C Activation Decreases Cell Surface Expression of the GLT-1 Subtype of Glutamate Transporter. REQUIREMENT OF A CARBOXYL-TERMINAL DOMAIN AND PARTIAL DEPENDENCE ON SERINE 486
J. Biol. Chem., November 22, 2002; 277(48): 45741 - 45750.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1999 by the American Society for Pharmacology and Experimental Therapeutics.