Vol. 289, Issue 1, 133-139, April 1999

Local -Bungarotoxin-Sensitive Nicotinic Receptors Modulate Hippocampal Norepinephrine Release by Systemic Nicotine¹

Yitong Fu, Shannon G. Matta and Burt M. Sharp

Department of Pharmacology, University of Tennessee, Memphis, Tennessee; and Minneapolis Medical Research Foundation, Minneapolis, Minnesota

Previous studies have shown that nicotinic receptors (NAChRs) accessible from the cerebral aqueduct of the brainstem mediate the hippocampal norepinephrine (NE) release induced by i.v. nicotine. The present study was designed to investigate the role of hippocampal NAChRs in this process. Nicotinic antagonists were microinjected or microdialyzed into the hippocampus (HP) before administering nicotine (0.09 mg/kg over 60 s, i.v.) to freely moving rats. -Bungarotoxin (0.3 nmol by microinjection) blocked nicotine-induced hippocampal NE release by 47% (p < .05) and abolished the effect of 0.065 mg/kg nicotine. Methyllycaconitine (1.4-5.6 mM in the dialysate) inhibited the stimulatory effect of nicotine 0.09 mg/kg by 48 to 75% (p < .05). In contrast, mecamylamine (2.9-5.8 mM) and dihydro--erythroidine (7-14 mM) were completely ineffective. The role of hippocampal NAChRs was demonstrated further by selectively desensitizing these receptors before the systemic infusion of nicotine. To do so, the HP was pretreated with nicotine (0.1 mM) delivered through the microdialysis probe; this concentration was calculated to yield tissue concentrations similar to those produced by the systemic infusions of nicotine. Dialyzing this concentration of nicotine into the HP inhibited the NE response to i.v. nicotine by 34% (p < .05), and 1.0 mM nicotine reduced the response by 40%. These studies indicate that -bungarotoxin-sensitive hippocampal NAChRs, probably containing 7 subunits, modulate hippocampal NE release because of systemic nicotine.