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Vol. 288, Issue 3, 1367-1373, March 1999
3-Adrenoceptor Subtypes in Relaxation
of the Human Urinary Bladder Detrusor: Analysis by Molecular Biological
and Pharmacological Methods1
Department of Urology, Niigata University School of Medicine,
Niigata, Japan (M.T., K.O., T.M., Y.T., K.A., T.T., A.H., K.T.); and
Department of Pathology, Osaka University School of Medicine, Osaka,
Japan (S.N.).
The purpose of the present study was to confirm the presence of
3-adrenoceptor subtype in the relaxation of human
urinary bladder detrusor tissue by reverse transcription-polymerase
chain reaction (PCR); direct sequencing of the PCR product, in situ hybridization; and isometric contraction. Using reverse
transcription-PCR, the mRNAs of three receptor subtypes
(1, 2, and 3) were
expressed in the human urinary bladder detrusor tissue. Direct
sequencing of the PCR product of the above
3-adrenoceptor revealed no mutation in the amplified
regions. In situ hybridization with digoxygenin-labeled oligonucleotide
probe revealed the presence of the mRNA of
3-adrenoceptor subtype in the smooth muscle of the
urinary bladder. The relaxant effects of isoproterenol (a nonselective
-adrenoceptor agonist); ZD7114, BRL37344, and CGP12177A (putative
selective 3-adrenoceptor agonists); and SR59230A (a
putative selective 3-adrenoceptor antagonist) were
tested using an isometric contraction technique. Isoproterenol in
either the presence or absence of both atenolol (a
1-adrenoceptor-selective antagonist) and butoxamine (a
2-adrenoceptor-selective antagonist) revealed a relaxant
effect on the carbachol-induced contraction of the human urinary
bladder detrusor. Both BRL37344 and CGP12177A also revealed relaxant
effects on the human urinary bladder detrusor, but ZD7114 did not
elicit any relaxation. These results suggest that
3-adrenoceptor may have some role in urine storage in
the human urinary bladder.
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