![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 288, Issue 1, 93-97, January 1999
Departments of Medicine and Physiology, Medical College of
Virginia, Virginia Commonwealth University, Richmond, Virginia
Previous studies have shown that the intestinal peristaltic reflex
initiated by mucosal stimulation is mediated by release of
5-hydroxytryptamine (HT) from enterochromaffin cells; 5-HT acts via
5-HT4 receptors in rat and human, and via both
5-HT4 and 5-HT3 receptors in guinea pig to
activate intramural sensory neurons that release calcitonin
gene-related peptide. In this study, selective agonists and antagonists
were used to examine the involvement of 5-HT4 and
5-HT3 receptors in colonic propulsion. The velocity of
propulsion was measured with artificial fecal pellets introduced into
the orad end of an isolated guinea pig colonic segment. Control
velocity ranged from 0.5 to 3.3 mm/s; mean ± S.E.M., 1.3 ± 0.1 mm/s. The 5-HT4 antagonist, GR 113808A, and the
5-HT3 antagonist, LY 278584, decreased the velocity of pellet propulsion in a concentration-dependent fashion (39 ± 2% and 47 ± 1% decrease at 10 µM, respectively). A combination of both antagonists (10 µM each) was additive, decreasing the velocity by 82 ± 3% to 84 ± 4%. The selective 5-HT4
agonists, HTF 919 and R093877, as well as 5-HT in the presence of the
5-HT2a antagonist, ketanserin, increased the velocity of
propulsion in a concentration-dependent fashion with EC50s
of 6.9 ± 0.1 nM, 37.4 ± 1.0 nM, and 3.9 ± 0.1 nM,
respectively. Compared with HTF 919, R093877 was less potent and
appeared to be a partial agonist. All three agonists were effective at
submicromolar concentrations; at concentrations above 1 µM, there was
no increase in the velocity of propulsion. We conclude that the
presence of fecal pellets triggers the release of 5-HT, which acts via
both 5-HT3 and 5-HT4 receptors to regulate propulsive activity in guinea pig colon.
This article has been cited by other articles:
|
|
 |
|
  E. J. Dickson, G. W. Hennig, D. J. Heredia, H.-T. Lee, P. O. Bayguinov, N. J. Spencer, and T. K. Smith Polarized intrinsic neural reflexes in response to colonic elongation J. Physiol., September 1, 2008; 586(17): 4225 - 4240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Lomax, J. R. O'Hara, N. P. Hyland, G. M. Mawe, and K. A. Sharkey Persistent alterations to enteric neural signaling in the guinea pig colon following the resolution of colitis Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G482 - G491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Grider and B. E. Piland The peristaltic reflex induced by short-chain fatty acids is mediated by sequential release of 5-HT and neuronal CGRP but not BDNF Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G429 - G437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, V. Martinez, H. Kimura, and Y. Tache 5-Hydroxytryptophan activates colonic myenteric neurons and propulsive motor function through 5-HT4 receptors in conscious mice Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G419 - G428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Grider Desensitization of the peristaltic reflex induced by mucosal stimulation with the selective 5-HT4 agonist tegaserod Am J Physiol Gastrointest Liver Physiol, February 1, 2006; 290(2): G319 - G327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liu, M. S. Geddis, Y. Wen, W. Setlik, and M. D. Gershon Expression and function of 5-HT4 receptors in the mouse enteric nervous system Am J Physiol Gastrointest Liver Physiol, December 1, 2005; 289(6): G1148 - G1163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kojima, T. Nakagawa, R. Katsui, H. Fujii, Y. Nakajima, and M. Takaki A 5-HT4 agonist, mosapride, enhances intrinsic rectorectal and rectoanal reflexes after removal of extrinsic nerves in guinea pigs Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G351 - G360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. O'Hara, W. Ho, D. R. Linden, G. M. Mawe, and K. A. Sharkey Enteroendocrine cells and 5-HT availability are altered in mucosa of guinea pigs with TNBS ileitis Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G998 - G1007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-C. Bian, L. F. Heffer, R. M. Gwynne, J. C. Bornstein, and P. P. Bertrand Synaptic transmission in simple motility reflex pathways excited by distension in guinea pig distal colon Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G1017 - G1027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sugiuar, K. Bielefeldt, and G. F. Gebhart TRPV1 Function in Mouse Colon Sensory Neurons Is Enhanced by Metabotropic 5-Hydroxytryptamine Receptor Activation J. Neurosci., October 27, 2004; 24(43): 9521 - 9530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F De Ponti Pharmacology of serotonin: what a clinician should know Gut, October 1, 2004; 53(10): 1520 - 1535. [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Mertz Irritable Bowel Syndrome N. Engl. J. Med., November 27, 2003; 349(22): 2136 - 2146. [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Grider Neurotransmitters Mediating the Intestinal Peristaltic Reflex in the Mouse J. Pharmacol. Exp. Ther., November 1, 2003; 307(2): 460 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tsubouchi, T. Saito, F. Mizutani, T. Yamauchi, and Y. Iwanaga Stimulatory Action of Itopride Hydrochloride on Colonic Motor Activity in Vitro and in Vivo J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 787 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shimatani, Y. Kojima, M. Kadowaki, T. Nakagawa, H. Fujii, Y. Nakajima, and M. Takaki A 5-HT4 agonist mosapride enhances rectorectal and rectoanal reflexes in guinea pigs Am J Physiol Gastrointest Liver Physiol, July 7, 2003; 285(2): G389 - G395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fukumoto, M. Tatewaki, T. Yamada, M. Fujimiya, C. Mantyh, M. Voss, S. Eubanks, M. Harris, T. N. Pappas, and T. Takahashi Short-chain fatty acids stimulate colonic transit via intraluminal 5-HT release in rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2003; 284(5): R1269 - R1276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Bush, N. J. Spencer, N. Watters, K. M. Sanders, and T. K. Smith Effects of alosetron on spontaneous migrating motor complexes in murine small and large bowel in vitro Am J Physiol Gastrointest Liver Physiol, October 1, 2001; 281(4): G974 - G983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Chen, Z. Li, H. Pan, D. L. Murphy, H. Tamir, H. Koepsell, and M. D. Gershon Maintenance of Serotonin in the Intestinal Mucosa and Ganglia of Mice that Lack the High-Affinity Serotonin Transporter: Abnormal Intestinal Motility and the Expression of Cation Transporters J. Neurosci., August 15, 2001; 21(16): 6348 - 6361. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
