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Vol. 287, Issue 3, 1119-1127, December 1998

Multiple Subtypes of Serotonin Receptors Are Expressed in Rat Sensory Neurons in Culture

Joanne J. Chen, Michael R. Vasko, Xiaoping Wu, Theodora P. Staeva, Melvyn Baez, John M. Zgombick and David L. Nelson

Neuroscience Research (J.J.C., M.B., D.L.N.), Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana; Department of Pharmacology and Toxicology (M.R.V., X.W., T.P.S.), Indiana University, School of Medicine, Indianapolis, Indiana; and Synaptic Pharmaceutical Corporation (J.M.Z.), Paramus, New Jersey

[3H]5-HT revealed the presence of serotonin receptors in cultured rat sensory neurons. [3H]5-CT binding was inhibited by cyanopindolol with an IC50 of 0.87 ± 0.30 nM, suggesting the expression of the 5-HT1B receptor in these neurons. The presence of 5-HT1B receptors was confirmed by the displacement of [125I]Iodocyanopindolol binding by cyanopindolol with an IC50 of 2.43 ± 0.81 nM. 5-HT1B receptors are the predominant type of serotonin receptors labeled by [3H]5-HT in cultured DRG neurons, representing ~60% of the specific [3H]5-HT binding sites. In addition, 5-HT1D and 5-HT2A receptor binding was also found in these neurons. RT-PCR analysis of RNA isolated from embryonic sensory neurons in culture confirmed the expression of 5-HT1B, 5-HT1D and 5-HT2A receptor mRNA. It also demonstrated the presence of 5-HT1F, 5-HT2C, 5-HT3, 5-HT4, 5-HT5A and 5-HT5B receptor mRNA and the absence of 5-HT1A, 5-HT1E, 5-HT2B, 5-HT6 and 5-HT7 mRNA. The identification of multiple subtypes of serotonin receptors expressed in cultured embryonic sensory neurons suggests that DRG neuronal cultures may be an excellent model to examine the direct effects of serotonin on the activity of these sensory neurons.

0022-3565/98/2873-1119$03.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 1998 by The American Society for Pharmacology and Experimental Therapeutics


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