![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 287, Issue 2, 648-657, November 1998
Institute for Behavioral Genetics, University of Colorado, Boulder,
Colorado
Several recent electrophysiological studies have demonstrated that
nicotinic agonists stimulate the release of 
-aminobutyric acid
(GABA) from rodent brain tissue. Our studies used a neurochemical approach to characterize nicotinic receptor-stimulated
[3H]-GABA release from mouse brain synaptosomes.
Nicotine increased [3H]-GABA release from synaptosomes
preloaded with [3H]-GABA in a concentration-dependent
manner. This release appeared rapidly, was Ca++ dependent,
and was partially (about 50%) blocked by 100 nM tetrodotoxin and
totally blocked by mecamylamine and dihydro-
-erythroidine. 
-Bungarotoxin had no effect. Twelve nicotinic agonists were compared for their effects on [3H]-GABA release. The agonists
differed in potency (EC50) and efficacy (Emax).
The EC50 and Emax values were significantly
correlated (r = 0.95, P < .001 for EC50; r = 0.93, P < .01 for Emax) to values obtained for
these same agonists when 86Rb+ efflux was
determined. A significant correlation (r = 0.84, P < .01)
was found when the EC50 values for agonist-stimulated
[3H]-GABA release and IC50 values for agonist
inhibition of [3H]-L-nicotine binding were
compared. Differences in [3H]-GABA release were detected
in 12 brain regions and maximal release was significantly correlated
with [3H]-nicotine binding. The pharmacological and
regional comparisons suggest that the nAChR that stimulates
[3H]-GABA release is the one that binds
[3H]-nicotine with high affinity (42). Unequivocal
evidence that the receptor that modulates nicotine-stimulated
[3H]-GABA release contains a 2 subunit was obtained in
a study using wild-type, heterozygous and homozygous 2 null mutant
mice. [3H]-GABA release and [3H]-nicotine
binding decreased along with the number of copies of the null mutant gene.
This article has been cited by other articles:
|
|
 |
|
  Y. Teper, D. Whyte, E. Cahir, H. A. Lester, S. R. Grady, M. J. Marks, B. N. Cohen, C. Fonck, T. McClure-Begley, J. M. McIntosh, et al. Nicotine-Induced Dystonic Arousal Complex in a Mouse Line Harboring a Human Autosomal-Dominant Nocturnal Frontal Lobe Epilepsy Mutation J. Neurosci., September 19, 2007; 27(38): 10128 - 10142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Salminen, J. A. Drapeau, J. M. McIntosh, A. C. Collins, M. J. Marks, and S. R. Grady Pharmacology of {alpha}-Conotoxin MII-Sensitive Subtypes of Nicotinic Acetylcholine Receptors Isolated by Breeding of Null Mutant Mice Mol. Pharmacol., June 1, 2007; 71(6): 1563 - 1571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-S. Chiu, S. Brickley, K. Jensen, A. Southwell, S. Mckinney, S. Cull-Candy, I. Mody, and H. A. Lester GABA Transporter Deficiency Causes Tremor, Ataxia, Nervousness, and Increased GABA-Induced Tonic Conductance in Cerebellum J. Neurosci., March 23, 2005; 25(12): 3234 - 3245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Jensen, C.-S. Chiu, I. Sokolova, H. A. Lester, and I. Mody GABA Transporter-1 (GAT1)-Deficient Mice: Differential Tonic Activation of GABAA Versus GABAB Receptors in the Hippocampus J Neurophysiol, October 1, 2003; 90(4): 2690 - 2701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Alkondon, E. F.R. Pereira, and E. X. Albuquerque NMDA and AMPA Receptors Contribute to the Nicotinic Cholinergic Excitation of CA1 Interneurons in the Rat Hippocampus J Neurophysiol, September 1, 2003; 90(3): 1613 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Dobelis, S. Hutton, Y. Lu, and A. C. Collins GABAergic Systems Modulate Nicotinic Receptor-Mediated Seizures in Mice J. Pharmacol. Exp. Ther., September 1, 2003; 306(3): 1159 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. King, M. J. Marks, S. R. Grady, B. J. Caldarone, A. O. Koren, A. G. Mukhin, A. C. Collins, and M. R. Picciotto Conditional Expression in Corticothalamic Efferents Reveals a Developmental Role for Nicotinic Acetylcholine Receptors in Modulation of Passive Avoidance Behavior J. Neurosci., May 1, 2003; 23(9): 3837 - 3843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Quik, J. D. Sum, P. Whiteaker, S. E. McCallum, M. J. Marks, J. Musachio, J. M. Mcintosh, A. C. Collins, and S. R. Grady Differential Declines in Striatal Nicotinic Receptor Subtype Function after Nigrostriatal Damage in Mice Mol. Pharmacol., May 1, 2003; 63(5): 1169 - 1179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-S. Chiu, K. Jensen, I. Sokolova, D. Wang, M. Li, P. Deshpande, N. Davidson, I. Mody, M. W. Quick, S. R. Quake, et al. Number, Density, and Surface/Cytoplasmic Distribution of GABA Transporters at Presynaptic Structures of Knock-In Mice Carrying GABA Transporter Subtype 1-Green Fluorescent Protein Fusions J. Neurosci., December 1, 2002; 22(23): 10251 - 10266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Köfalvi, B. Sperlágh, T. Zelles, and E. S. Vizi Long-Lasting Facilitation of 4-Amino-n-[2,3-3H]butyric Acid ([3H]GABA) Release from Rat Hippocampal Slices by Nicotinic Receptor Activation J. Pharmacol. Exp. Ther., November 1, 2000; 295(2): 453 - 462. [Abstract] [Full Text]
|
|
|
|
|
|
J. A. Stitzel, M. Jimenez, M. J. Marks, T. Tritto, and A. C. Collins Potential Role of the alpha 4 and alpha 6 Nicotinic Receptor Subunits in Regulating Nicotine-Induced Seizures J. Pharmacol. Exp. Ther., April 1, 2000; 293(1): 67 - 74. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. S. Vizi Role of High-Affinity Receptors and Membrane Transporters in Nonsynaptic Communication and Drug Action in the Central Nervous System Pharmacol. Rev., March 1, 2000; 52(1): 63 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Alkondon, E. F. R. Pereira, H. M. Eisenberg, and E. X. Albuquerque Nicotinic Receptor Activation in Human Cerebral Cortical Interneurons: a Mechanism for Inhibition and Disinhibition of Neuronal Networks J. Neurosci., January 1, 2000; 20(1): 66 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lu, M. J. Marks, and A. C. Collins Desensitization of Nicotinic Agonist-Induced [3H]gamma -Aminobutyric Acid Release from Mouse Brain Synaptosomes Is Produced by Subactivating Concentrations of Agonists J. Pharmacol. Exp. Ther., December 1, 1999; 291(3): 1127 - 1134. [Abstract] [Full Text]
|
|
|
|
|
|
M. Alkondon, E. F. R. Pereira, H. M. Eisenberg, and E. X. Albuquerque Choline and Selective Antagonists Identify Two Subtypes of Nicotinic Acetylcholine Receptors that Modulate GABA Release from CA1 Interneurons in Rat Hippocampal Slices J. Neurosci., April 1, 1999; 19(7): 2693 - 2705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. McIntosh, G. O. Corpuz, R. T. Layer, J. E. Garrett, J. D. Wagstaff, G. Bulaj, A. Vyazovkina, D. Yoshikami, L. J. Cruz, and B. M. Olivera Isolation and Characterization of a Novel Conus Peptide with Apparent Antinociceptive Activity J. Biol. Chem., October 13, 2000; 275(42): 32391 - 32397. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
