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Vol. 284, Issue 1, 399-405, 1998
Department of Pharmacology and Cardiovascular Research Institute
Maastricht (CARIM), Universiteit Maastricht, Maastricht, The
Netherlands
We evaluated the relationship between the presence of adrenergic nerves
and the presence of alpha-1 adrenoceptors
(alpha-1 AR) in the arterial tree of the rat. The thoracic
aorta and the carotid, mammary, renal and femoral arteries were
isolated from 20-week-old male WKY rats, along with the superior
mesenteric artery and small (first order) and resistance-sized (third
order) side branches of this vessel. Norepinephrine content ([NE])
and specific binding of 300 pM [3H]prazosin were
determined. To estimate the total density of alpha-1 AR
([alpha-1 AR]) as well as the density of
alpha-1A AR ([alpha-1A AR]), binding
experiments were performed with and without pretreatment of the
preparations with the irreversible alpha-1B AR and
alpha-1D AR antagonist chloroethylclonidine and in the
absence and presence of the alpha-1A AR selective ligand
(+)-niguldipine (30 nM). Also the presence of mRNA for
alpha-1A AR was evaluated by use of reverse transcriptase-polymerase chain reaction (RT-PCR). In intact rats, arterial [NE] ranged between 0.1 and 15 ng/µg DNA, arterial
[alpha-1 AR] ranged between 12.4 and 46.8 fmol/mg protein
and [alpha-1A AR] ranged between 0.05 and 27.9 fmol/mg
protein. There was no significant correlation between
[alpha-1 AR] and [NE]. However, with respect to the
[alpha-1A AR] a significant correlation between [NE] and
[alpha-1A AR] was observed. RT-PCR analysis confirmed the
expression of alpha-1A AR in the densely innervated
mesenteric resistance-sized arteries. Two weeks after chemical
sympathectomy of the rats with 6-hydroxydopamine (i) arterial [NE]
was markedly reduced, and (ii) a distinct reduction in the
[alpha-1A AR] as percentage of the total
[alpha-1 AR] density in mesenteric artery side branches
was noted. These findings indicate that there is a positive and
reversible relationship between the presence of adrenergic nerves and
that of alpha-1A AR in rat arteries.
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