![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 283, Issue 3, 1293-1304, 1997
Department of Pharmacology, Chung Ang University Pharmacy College,
Seoul 156-756, Korea (U.D.S.),
Department of Medicine, Rhode Island
Hospital and Brown University School of Medicine, Providence, Rhode
Island (K.M.H., W.C., J.B., P.B),
Department of Medicine, VA Medical
Center and Brown University School of Medicine, Providence, Rhode
Island (H.R.) and
Kangnam General Hospital, Public Corporation, Seoul,
135-090, Korea (N.K.)
In single cells, isolated by enzymatic digestion from the circular
muscle layer of the lower esophageal sphincter (LES), acute experimental esophagitis (AE) alters signal transduction in response to
a maximally effective dose of acetylcholine. In normal LES contraction
was inhibited by M3 
M1 or M2
antagonists. In AE inhibition by M2 antagonists increased
significantly so that contraction was inhibited by M3 > M2 > M1 antagonists. In normal cells
permeabilized by saponin, contraction was antagonized by antibodies
against Gq/11, by the phosphatidylinositol-specific
phospholipase C (PI-PLC) antagonist U 73122, but not by the
phosphatidylcholine-specific phospholipase C (PC-PLC) inhibitor D609,
or by the phospholipase D pathway inhibitor propranolol. In AE
contraction was reduced by Gq/11 and Gi3
antibodies and by U73122, propranolol and D609. After thapsigargin
treatment of normal cells to reduce intracellular Ca++
stores, contraction was inhibited by M2 and M3
antagonists, by antibodies against Gq/11 and
Gi3, by U73122, D609 and propranolol, suggesting that
depletion of Ca++ stores reproduces the changes induced by
AE. We conclude that in normal LES smooth muscle cells
acetylcholine-induced contraction is mediated by M3
receptors linked to Gq/11 and PI-PLC, whereas in AE,
contraction through this pathway is reduced, perhaps because of
reduction in Ca++ stores, and a second pathway is activated
by M2 receptors linked to Gi3, PC-PLC and
phospholipase D.
This article has been cited by other articles:
|
|
 |
|
  R W Wells and M G Blennerhassett Divergent changes to muscarinic and serotonergic signalling following colitis Gut, December 1, 2005; 54(12): 1699 - 1706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Cheng, W. Cao, C. Fiocchi, J. Behar, P. Biancani, and K. M. Harnett In vitro model of acute esophagitis in the cat Am J Physiol Gastrointest Liver Physiol, November 1, 2005; 289(5): G860 - G869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Cao, K. M. Harnett, L. Cheng, M. T. Kirber, J. Behar, and P. Biancani H2O2: a mediator of esophagitis-induced damage to calcium-release mechanisms in cat lower esophageal sphincter Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1170 - G1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cheng, W. Cao, J. Behar, P. Biancani, and K. M. Harnett Inflammation induced changes in arachidonic acid metabolism in cat LES circular muscle Am J Physiol Gastrointest Liver Physiol, April 1, 2005; 288(4): G787 - G797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Harnett, W. Cao, and P. Biancani Signal-Transduction Pathways that Regulate Smooth Muscle Function I. Signal transduction in phasic (esophageal) and tonic (gastroesophageal sphincter) smooth muscles Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G407 - G416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Song, T. S. Lee, J. H. Jeong, Y. S. Min, C. Y. Shin, and U. D. Sohn Hydrogen Peroxide-Induced Extracellular Signal-Regulated Kinase Activation in Cultured Feline Ileal Smooth Muscle Cells J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 391 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Cao, M. D. Vrees, F. M. Potenti, K. M. Harnett, C. Fiocchi, and V. E. Pricolo Interleukin 1{beta}-Induced Production of H2O2 Contributes to Reduced Sigmoid Colonic Circular Smooth Muscle Contractility in Ulcerative Colitis J. Pharmacol. Exp. Ther., October 1, 2004; 311(1): 60 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Z. Shi and S. K. Sarna G protein-mediated dysfunction of excitation-contraction coupling in ileal inflammation Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G899 - G905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Pontari, A. S. Braverman, and M. R. Ruggieri Sr. The M2 muscarinic receptor mediates in vitro bladder contractions from patients with neurogenic bladder dysfunction Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2004; 286(5): R874 - R880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Braverman and M. R. Ruggieri Sr. Hypertrophy changes the muscarinic receptor subtype mediating bladder contraction from M3 toward M2 Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2003; 285(3): R701 - R708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Sarna Neuronal locus and cellular signaling for stimulation of ileal giant migrating and phasic contractions Am J Physiol Gastrointest Liver Physiol, May 1, 2003; 284(5): G789 - G797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Shin, Y. P. Lee, T. S. Lee, H. D. Je, D. S. Kim, and U. D. Sohn The Signal Transduction of Endothelin-1-Induced Circular Smooth Muscle Cell Contraction in Cat Esophagus J. Pharmacol. Exp. Ther., September 1, 2002; 302(3): 924 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Braverman, R. J. Tallarida, and M. R. Ruggieri Sr. Interaction between muscarinic receptor subtype signal transduction pathways mediating bladder contraction Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R663 - R668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. D. Sohn, W. Cao, D.-C. Tang, J. T. Stull, J. R. Haeberle, C.-L. A. Wang, K. M. Harnett, J. Behar, and P. Biancani Myosin light chain kinase- and PKC-dependent contraction of LES and esophageal smooth muscle Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G467 - G478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-L. Xiao, Q. Chen, P. Biancani, and J. Behar Abnormalities of gallbladder muscle associated with acute inflammation in guinea pigs Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G490 - G497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S D Smid and L A Blackshaw Neuromuscular function of the human lower oesophageal sphincter in reflux disease and Barrett's oesophagus Gut, June 1, 2000; 46(6): 756 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. B. Cao, K. M. Harnett, Q. Chen, M. K. Jain, J. Behar, and P. Biancani Group I secreted PLA2 and arachidonic acid metabolites in the maintenance of cat LES tone Am J Physiol Gastrointest Liver Physiol, September 1, 1999; 277(3): G585 - G598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-P. Fan, S. Chakder, and S. Rattan Mechanism of action of cholera toxin on the opossum internal anal sphincter smooth muscle Am J Physiol Gastrointest Liver Physiol, July 1, 1999; 277(1): G152 - G160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. P. Parkman, A. P. Pagano, and J. P. Ryan Subtypes of muscarinic receptors regulating gallbladder cholinergic contractions Am J Physiol Gastrointest Liver Physiol, May 1, 1999; 276(5): G1243 - G1250. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
