JPET Celsis microsomes equal better data

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Conner, E. M.
Right arrow Articles by Grisham, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Conner, E. M.
Right arrow Articles by Grisham, M. B.

Vol. 282, Issue 3, 1615-1622, 1997

Proteasome Inhibition Attenuates Nitric Oxide Synthase Expression, VCAM-1 Transcription and the Development of Chronic Colitis1

Elaine M. Conner, Stephen Brand, Jonathan M. Davis, F. Stephen Laroux, Vito J. Palombella, John W. Fuseler, David Y. Kang, Robert E. Wolf and Matthew B. Grisham

Departments of Molecular and Cellular Physiology (E.M.C., J.M.D., F.S.L., D.Y.K., M.B.G.) and Medicine (J.W.F., R.E.W.), Louisiana State University Medical Center, Shreveport, Louisiana, and ProScript, Inc., Cambridge, Massachusetts (S.B., V.J.P).

The objectives of this study were to (1) assess the role of the 26S proteasome complex in regulating the expression of the inducible isoform of nitric oxide synthase (iNOS) and vascular cell adhesion molecule-1 (VCAM-1) in a model of chronic granulomatous colitis in vivo and (2) determine the role of the proteasome in regulating the inflammatory response observed in this model of chronic gut inflammation. The selective proteasome inhibitor MG-341 (0.3 mg/kg) was administered by gavage beginning immediately before the induction of colitis and continuing daily thereafter for the entire 14-day experimental period. We found that chronic proteasome inhibition using MG-341 significantly attenuated the peptidoglycan/polysaccharide (PG/PS)-induced up-regulation of iNOS in the colon and spleen and the consequent increase in plasma levels of nitrate and nitrite. Furthermore, we found that the proteasome inhibitor suppressed the up-regulation of the adhesion molecule VCAM-1 in the colon. We also found that MG-341 attenuated PG/PS-induced increases in macroscopic colonic inflammation, bowel wall thickness, colonic dry weight and colonic MPO activity. Treatment with MG-341 also significantly reduced PG/PS-induced increases in macroscopic spleen inflammation, spleen weight and spleen MPO activity. We conclude that the 26S proteasome complex plays an important role in regulating the PG/PS-induced up-regulation of iNOS and VCAM-1 in vivo and appears to be important in regulating colonic and splenic inflammation.

Copyright © by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
H. Kim, H. Jeon, H. Kong, Y. Yang, B. Choi, Y. M. Kim, L. Neckers, and Y. Jung
A Molecular Mechanism for the Anti-Inflammatory Effect of Taurine-Conjugated 5-Aminosalicylic Acid in Inflamed Colon
Mol. Pharmacol., April 1, 2006; 69(4): 1405 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
K. P. Pavlick, D. V. Ostanin, K. L. Furr, F. S. Laroux, C. M. Brown, L. Gray, C. G. Kevil, and M. B. Grisham
Role of T-cell-associated lymphocyte function-associated antigen-1 in the pathogenesis of experimental colitis
Int. Immunol., February 1, 2006; 18(2): 389 - 398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D. V. Ostanin, K. P. Pavlick, S. Bharwani, D. D'Souza, K. L. Furr, C. M. Brown, and M. B. Grisham
T cell-induced inflammation of the small and large intestine in immunodeficient mice
Am J Physiol Gastrointest Liver Physiol, January 1, 2006; 290(1): G109 - G119.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. M. Dagia, N. Harii, A. E. Meli, X. Sun, C. J. Lewis, L. D. Kohn, and D. J. Goetz
Phenyl Methimazole Inhibits TNF-{alpha}-Induced VCAM-1 Expression in an IFN Regulatory Factor-1-Dependent Manner and Reduces Monocytic Cell Adhesion to Endothelial Cells
J. Immunol., August 1, 2004; 173(3): 2041 - 2049.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
F. S. Laroux, H. H. Norris, J. Houghton, K. P. Pavlick, S. Bharwani, D. M. Merrill, J. Fuseler, R. Chervenak, and M. B. Grisham
Regulation of chronic colitis in athymic nu/nu (nude) mice
Int. Immunol., January 1, 2004; 16(1): 77 - 89.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. B. Mehta and G. E. Besner
Inhibition of NF-{kappa}B Activation and Its Target Genes by Heparin-Binding Epidermal Growth Factor-Like Growth Factor
J. Immunol., December 1, 2003; 171(11): 6014 - 6022.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. M. Dagia and D. J. Goetz
A proteasome inhibitor reduces concurrent, sequential, and long-term IL-1{beta}- and TNF-{alpha}-induced ECAM expression and adhesion
Am J Physiol Cell Physiol, October 1, 2003; 285(4): C813 - C822.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. A. Pritts, E. S. Hungness, D. D. Hershko, B. W. Robb, X. Sun, G.-J. Luo, J. E. Fischer, H. R. Wong, and P.-O. Hasselgren
Proteasome inhibitors induce heat shock response and increase IL-6 expression in human intestinal epithelial cells
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R1016 - R1026.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C. F. Krieglstein, W. H. Cerwinka, F. S. Laroux, J. W. Salter, J. M. Russell, G. Schuermann, M. B. Grisham, C. R. Ross, and D. N. Granger
Regulation of Murine Intestinal Inflammation by Reactive Metabolites of Oxygen and Nitrogen: Divergent Roles of Superoxide and Nitric Oxide
J. Exp. Med., October 29, 2001; 194(9): 1207 - 1218.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Oshima, K. P. Pavlick, F. S. Laroux, S. K. Verma, P. Jordan, M. B. Grisham, L. Williams, and J. S. Alexander
Regulation and distribution of MAdCAM-1 in endothelial cells in vitro
Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1096 - C1105.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
J. B. Phillips, A. J. Williams, J. Adams, P. J. Elliott, F. C. Tortella, and J. A. Clemens
Proteasome Inhibitor PS519 Reduces Infarction and Attenuates Leukocyte Infiltration in a Rat Model of Focal Cerebral Ischemia Editorial Comment
Stroke, July 1, 2000; 31(7): 1686 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Russell, C. J. Epstein, M. B. Grisham, J. S. Alexander, K.-Y. Yeh, and D. N. Granger
Regulation of E-selectin expression in postischemic intestinal microvasculature
Am J Physiol Gastrointest Liver Physiol, June 1, 2000; 278(6): G878 - G885.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Kawachi, S. Jennings, J. Panes, A. Cockrell, F. S. Laroux, L. Gray, M. Perry, H. van der Heyde, E. Balish, D. N. Granger, et al.
Cytokine and endothelial cell adhesion molecule expression in interleukin-10-deficient mice
Am J Physiol Gastrointest Liver Physiol, May 1, 2000; 278(5): G734 - G743.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Kawachi, A. Cockrell, F. S. Laroux, L. Gray, D. N. Granger, H. C. van der Heyde, and M. B. Grisham
Role of inducible nitric oxide synthase in the regulation of VCAM-1 expression in gut inflammation
Am J Physiol Gastrointest Liver Physiol, September 1, 1999; 277(3): G572 - G576.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. J. Kalogeris, F. S. Laroux, A. Cockrell, H. Ichikawa, N. Okayama, T. J. Phifer, J. S. Alexander, and M. B. Grisham
Effect of selective proteasome inhibitors on TNF-induced activation of primary and transformed endothelial cells
Am J Physiol Cell Physiol, April 1, 1999; 276(4): C856 - C864.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. J. Palombella, E. M. Conner, J. W. Fuseler, A. Destree, J. M. Davis, F. S. Laroux, R. E. Wolf, J. Huang, S. Brand, P. J. Elliott, et al.
Role of the proteasome and NF-kappa B in streptococcal cell wall-induced polyarthritis
PNAS, December 22, 1998; 95(26): 15671 - 15676.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1997 by the American Society for Pharmacology and Experimental Therapeutics.