![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 282, Issue 3, 1358-1365, 1997
Department of Pharmacology, University of Colorado Health Sciences
Center, Denver, Colorado (S.J.Z., N.R.Z.) and
Vollum Institute,
Oregon Health Sciences University, Portland, Oregon (M.P.K., M.S.S.,
S.G.A.)
Activation of protein kinase C (PKC) regulates the activity of a number
of neurotransmitter transporters. When Xenopus oocytes expressing the cloned human dopamine transporter (hDAT) were pretreated with bath-applied phorbol 12-myristate 13-acetate (PMA), a PKC activator, [3H]DA uptake decreased irreversibly in a
time- and dose-dependent manner (IC50 = 22 nM; maximal
inhibition = 63-85%). The inhibition appeared to be PKC-specific
because incubation with the inactive form of phorbol ester
4
-phorbol-12,13-didecanoate (400 nM) did not change the uptake
activity and PMA (100 nM) inhibition could be partially blocked by the
selective PKC inhibitor bisindolylmaleimide I (1 µM). Saturation
studies of [3H]DA uptake showed that PMA-induced
inhibition was due to a decrease in Vmax with no change in
KT. Similar to uptake, PMA pretreatment inhibited both the
hDAT transport-associated and substrate-independent leak currents. PMA
also decreased membrane capacitance (Cm) by 40%,
selectively in hDAT-expressing oocytes. In addition, PMA pretreatment
resulted in a 77% decrease in Bmax of
[3H]mazindol binding to intact oocytes. In contrast,
binding to whole homogenates of PMA-pretreated oocytes was not
significantly altered. These results suggest that PMA regulates hDAT
expressed in Xenopus oocytes by altering cell surface
trafficking of hDAT.
This article has been cited by other articles:
|
|
 |
|
  M. Mertl, H. Daniel, and G. Kottra Substrate-induced changes in the density of peptide transporter PEPT1 expressed in Xenopus oocytes Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1332 - C1343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miranda, K. R. Dionne, T. Sorkina, and A. Sorkin Three Ubiquitin Conjugation Sites in the Amino Terminus of the Dopamine Transporter Mediate Protein Kinase C-dependent Endocytosis of the Transporter Mol. Biol. Cell, January 1, 2007; 18(1): 313 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Kahlig, B. J. Lute, Y. Wei, C. J. Loland, U. Gether, J. A. Javitch, and A. Galli Regulation of Dopamine Transporter Trafficking by Intracellular Amphetamine Mol. Pharmacol., August 1, 2006; 70(2): 542 - 548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Perry, J. Blaine, H. Le, and I. I. Grichtchenko PMA- and ANG II-induced PKC regulation of the renal Na+-HCO3- cotransporter (hkNBCe1) Am J Physiol Renal Physiol, February 1, 2006; 290(2): F417 - F427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miranda, C. C. Wu, T. Sorkina, D. R. Korstjens, and A. Sorkin Enhanced Ubiquitylation and Accelerated Degradation of the Dopamine Transporter Mediated by Protein Kinase C J. Biol. Chem., October 21, 2005; 280(42): 35617 - 35624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Blakely, L. J. DeFelice, and A. Galli Biogenic Amine Neurotransmitter Transporters: Just When You Thought You Knew Them Physiology, August 1, 2005; 20(4): 225 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Backs, E. Bresch, M. Lutz, A. V. Kristen, and M. Haass Endothelin-1 inhibits the neuronal norepinephrine transporter in hearts of male rats Cardiovasc Res, August 1, 2005; 67(2): 283 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. G. Garcia, Y. Wei, J. A. Moron, R. Z. Lin, J. A. Javitch, and A. Galli Akt Is Essential for Insulin Modulation of Amphetamine-Induced Human Dopamine Transporter Cell-Surface Redistribution Mol. Pharmacol., July 1, 2005; 68(1): 102 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Kahlig, J. A. Javitch, and A. Galli Amphetamine Regulation of Dopamine Transport: COMBINED MEASUREMENTS OF TRANSPORTER CURRENTS AND TRANSPORTER IMAGING SUPPORT THE ENDOCYTOSIS OF AN ACTIVE CARRIER J. Biol. Chem., March 5, 2004; 279(10): 8966 - 8975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hagos, B. C. Burckhardt, A. Larsen, C. Mathys, T. Gronow, A. Bahn, N. A. Wolff, G. Burckhardt, and J. Steffgen Regulation of sodium-dicarboxylate cotransporter-3 from winter flounder kidney by protein kinase C Am J Physiol Renal Physiol, January 1, 2004; 286(1): F86 - F93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Chi and M. E. A. Reith Substrate-Induced Trafficking of the Dopamine Transporter in Heterologously Expressing Cells and in Rat Striatal Synaptosomal Preparations J. Pharmacol. Exp. Ther., November 1, 2003; 307(2): 729 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Moron, I. Zakharova, J. V. Ferrer, G. A. Merrill, B. Hope, E. M. Lafer, Z. C. Lin, J. B. Wang, J. A. Javitch, A. Galli, et al. Mitogen-Activated Protein Kinase Regulates Dopamine Transporter Surface Expression and Dopamine Transport Capacity J. Neurosci., September 17, 2003; 23(24): 8480 - 8488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Loder and H. E. Melikian The Dopamine Transporter Constitutively Internalizes and Recycles in a Protein Kinase C-regulated Manner in Stably Transfected PC12 Cell Lines J. Biol. Chem., June 6, 2003; 278(24): 22168 - 22174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Granas, J. Ferrer, C. J. Loland, J. A. Javitch, and U. Gether N-terminal Truncation of the Dopamine Transporter Abolishes Phorbol Ester- and Substance P Receptor-stimulated Phosphorylation without Impairing Transporter Internalization J. Biol. Chem., February 7, 2003; 278(7): 4990 - 5000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-J. Liang, D. Johnson, L.-S. Ma, and S. M. Jarvis Regulation of the human vitamin C transporters expressed in COS-1 cells by protein kinase C Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1696 - C1704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Stevens, D. H. Feldman, Z. Liu, and W. R. Harvey Conserved tyrosine-147 plays a critical role in the ligand-gated current of the epithelial cation/amino acid transporter/channel CAATCH1 J. Exp. Biol., August 15, 2002; 205(16): 2545 - 2553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-N. Huang and P. W. Swaan Riboflavin Uptake in Human Trophoblast-Derived BeWo Cell Monolayers: Cellular Translocation and Regulatory Mechanisms J. Pharmacol. Exp. Ther., July 1, 2001; 298(1): 264 - 271. [Abstract] [Full Text]
|
|
|
|
|
|
L. Kantor, G. H. K. Hewlett, Y. H. Park, S. M. Richardson-Burns, M. J. Mellon, and M. E. Gnegy Protein Kinase C and Intracellular Calcium Are Required for Amphetamine-Mediated Dopamine Release via the Norepinephrine Transporter in Undifferentiated PC12 Cells J. Pharmacol. Exp. Ther., June 1, 2001; 297(3): 1016 - 1024. [Abstract] [Full Text]
|
|
|
|
|
|
S. Doolen and N. R. Zahniser Protein Tyrosine Kinase Inhibitors Alter Human Dopamine Transporter Activity in Xenopus Oocytes J. Pharmacol. Exp. Ther., March 1, 2001; 296(3): 931 - 938. [Abstract] [Full Text]
|
|
|
|
|
|
R. D. Mayfield and N. R. Zahniser Dopamine D2 Receptor Regulation of the Dopamine Transporter Expressed in Xenopus laevis Oocytes Is Voltage-Independent Mol. Pharmacol., January 1, 2001; 59(1): 113 - 121. [Abstract] [Full Text]
|
|
|
|
|
|
R. R. Metzger, H. M. Haughey, D. G. Wilkins, J. W. Gibb, G. R. Hanson, and A. E. Fleckenstein Methamphetamine-Induced Rapid Decrease in Dopamine Transporter Function: Role of Dopamine and Hyperthermia J. Pharmacol. Exp. Ther., December 1, 2000; 295(3): 1077 - 1085. [Abstract] [Full Text]
|
|
|
|
|
|
A. L. Bauman, S. Apparsundaram, S. Ramamoorthy, B. E. Wadzinski, R. A. Vaughan, and R. D. Blakely Cocaine and Antidepressant-Sensitive Biogenic Amine Transporters Exist in Regulated Complexes with Protein Phosphatase 2A J. Neurosci., October 15, 2000; 20(20): 7571 - 7578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Bian, W.-M. Zhang, J.-M. Pei, and T.-M. Wong The Role of Phosphodiesterase in Mediating the Effect of Protein Kinase C on Cyclic AMP Accumulation upon kappa -Opioid Receptor Stimulation in the Rat Heart J. Pharmacol. Exp. Ther., March 1, 2000; 292(3): 1065 - 1070. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. S. Vizi Role of High-Affinity Receptors and Membrane Transporters in Nonsynaptic Communication and Drug Action in the Central Nervous System Pharmacol. Rev., March 1, 2000; 52(1): 63 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Ingram and S. G. Amara Arachidonic Acid Stimulates a Novel Cocaine-Sensitive Cation Conductance Associated with the Human Dopamine Transporter J. Neurosci., January 15, 2000; 20(2): 550 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Daniels and S. G. Amara Regulated Trafficking of the Human Dopamine Transporter. CLATHRIN-MEDIATED INTERNALIZATION AND LYSOSOMAL DEGRADATION IN RESPONSE TO PHORBOL ESTERS J. Biol. Chem., December 10, 1999; 274(50): 35794 - 35801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. E. Melikian and K. M. Buckley Membrane Trafficking Regulates the Activity of the Human Dopamine Transporter J. Neurosci., September 15, 1999; 19(18): 7699 - 7710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ramamoorthy and R. D. Blakely Phosphorylation and Sequestration of Serotonin Transporters Differentially Modulated by Psychostimulants Science, July 30, 1999; 285(5428): 763 - 766. [Abstract] [Full Text]
|
|
|
|
|
|
M. Batchelor and J. O. Schenk Protein Kinase A Activity May Kinetically Upregulate the Striatal Transporter for Dopamine J. Neurosci., December 15, 1998; 18(24): 10304 - 10309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Apparsundaram, A. Galli, L. J. DeFelice, H. C. Hartzell, and R. D. Blakely Acute Regulation of Norepinephrine Transport: I. Protein Kinase C-Linked Muscarinic Receptors Influence Transport Capacity and Transporter Density in SK-N-SH Cells J. Pharmacol. Exp. Ther., November 1, 1998; 287(2): 733 - 743. [Abstract] [Full Text]
|
|
|
|
|
|
S. Apparsundaram, S. Schroeter, E. Giovanetti, and R. D. Blakely Acute Regulation of Norepinephrine Transport: II. PKC-Modulated Surface Expression of Human Norepinephrine Transporter Proteins J. Pharmacol. Exp. Ther., November 1, 1998; 287(2): 744 - 751. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Galli, R. D. Blakely, and L. J. DeFelice Patch-clamp and amperometric recordings from norepinephrine transporters: Channel activity and voltage-dependent uptake PNAS, October 27, 1998; 95(22): 13260 - 13265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Davis, D. J. Straff, E. A. Weinstein, P. G. Bannerman, D. M. Correale, J. D. Rothstein, and M. B. Robinson Multiple Signaling Pathways Regulate Cell Surface Expression and Activity of the Excitatory Amino Acid Carrier 1 Subtype of Glu Transporter in C6 Glioma J. Neurosci., April 1, 1998; 18(7): 2475 - 2485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Saunders, J. V. Ferrer, L. Shi, J. Chen, G. Merrill, M. E. Lamb, L. M. F. Leeb-Lundberg, L. Carvelli, J. A. Javitch, and A. Galli Amphetamine-induced loss of human dopamine transporter activity: An internalization-dependent and cocaine-sensitive mechanism PNAS, June 6, 2000; 97(12): 6850 - 6855. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
