![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 282, Issue 2, 1109-1116, 1997
Laboratory of Pharmacology and Chemistry, National Institutes of
Health, National Institute of Environmental Health Sciences, Research
Triangle Park, North Carolina (A.R.V., J.B.P.); and
Department of
Clinical Neurosciences, Brown University/Rhode Island Hospital,
Providence, Rhode Island (J.T.P.)
The objective of this study was to develop and evaluate a primary
culture system for choroid plexus epithelial cells as an in
vitro model for studying organic cation transport. Cells were dispersed from choroid plexus of neonatal rats by enzymatic digestion and grew as differentiated monolayers when plated on solid or permeable
support. Electron microscopy showed that cultured cells were
morphologically similar to intact choroid plexus epithelium, having
apical tight junctions between cells, numerous mitochondria, basal
nuclei and apical microvilli and cilia. As previously demonstrated for
intact choroid plexus, immunocytochemistry showed that
Na+,K+-ATPase was localized to the apical
membrane, and GLUT-1, the facilitative glucose transporter, was
localized to the basolateral membrane of cultured cells. Apical
transport of L-proline by cultured cells was mediated by a
sodium-dependent, electrogenic process, as in whole tissue.
14C-Tetraethylammonium (TEA), a prototypic organic cation,
was accumulated by isolated choroid plexus in a time-dependent manner;
uptake was inhibited by tetrapentyl-ammonium (TePA). In cultured cells, apical TEA transport was mediated by a saturable process coupled to
cellular metabolism. Unlabeled TEA and other organic cations (TePA,
N1-methylnicotinamide and mepiperphenidol) inhibited TEA
transport; the organic anion, p-aminohippurate, had no
effect. Finally, TePA-sensitive transport of 14C-TEA was
stimulated after preloading the cells with unlabeled TEA. Based on the
morphological, biochemical and functional properties of these cultured
cells, we conclude that this primary culture system should be an
excellent in vitro model for experimental characterization
of choroid plexus function.
This article has been cited by other articles:
|
|
 |
|
  V. Reichel, D. S. Miller, and G. Fricker Texas Red transport across rat and dogfish shark (Squalus acanthias) choroid plexus Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2008; 295(4): R1311 - R1319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Baehr, G. Fricker, and D. S. Miller Fluorescein-methotrexate transport in dogfish shark (Squalus acanthias) choroid plexus Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2006; 291(2): R464 - R472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nagata, H. Kusuhara, S. Hirono, H. Endou, and Y. Sugiyama CARRIER-MEDIATED UPTAKE OF H2-RECEPTOR ANTAGONISTS BY THE RAT CHOROID PLEXUS: INVOLVEMENT OF RAT ORGANIC ANION TRANSPORTER 3 Drug Metab. Dispos., September 1, 2004; 32(9): 1040 - 1047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Sweet, D. S. Miller, J. B. Pritchard, Y. Fujiwara, D. R. Beier, and S. K. Nigam Impaired Organic Anion Transport in Kidney and Choroid Plexus of Organic Anion Transporter 3 (Oat3 (Slc22a8)) Knockout Mice J. Biol. Chem., July 19, 2002; 277(30): 26934 - 26943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Lee, S. Dallas, M. Hong, and R. Bendayan Drug Transporters in the Central Nervous System: Brain Barriers and Brain Parenchyma Considerations Pharmacol. Rev., December 1, 2001; 53(4): 569 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Pritchard, D. H. Sweet, D. S. Miller, and R. Walden Mechanism of Organic Anion Transport across the Apical Membrane of Choroid Plexus J. Biol. Chem., November 19, 1999; 274(47): 33382 - 33387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Strazielle and J.-F. Ghersi-Egea Demonstration of a Coupled Metabolism-Efflux Process at the Choroid Plexus as a Mechanism of Brain Protection Toward Xenobiotics J. Neurosci., August 1, 1999; 19(15): 6275 - 6289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Villalobos, J. T. Parmelee, and J. L. Renfro Choline uptake across the ventricular membrane of neonate rat choroid plexus Am J Physiol Cell Physiol, June 1, 1999; 276(6): C1288 - C1296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Miller, A. R. Villalobos, and J. B. Pritchard Organic cation transport in rat choroid plexus cells studied by fluorescence microscopy Am J Physiol Cell Physiol, April 1, 1999; 276(4): C955 - C968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. V. Rao, J. L. Dahlheimer, M. E. Bardgett, A. Z. Snyder, R. A. Finch, A. C. Sartorelli, and D. Piwnica-Worms Choroid plexus epithelial expression of MDR1 P glycoprotein and multidrug resistance-associated protein contribute to the blood-cerebrospinal-fluid drug-permeability barrier PNAS, March 30, 1999; 96(7): 3900 - 3905. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
